Rnaextraction

RNA Extraction

We are pleased to offer two methods of RNA extraction. Both methods retain total RNA, including small RNAs.

Qiagen miRNeasy

For most low- to medium-throughput experiments, we recommend the column-based Qiagen miRNeasy.

https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/rna-purification/mirna/mirneasy-kits?catno=217004

This kit purifies RNA from up to 5 mg tissue, or cells (up to 1 x 106 cells).

Samples should be provided to the Genome Core frozen in exactly 700 ul of Qiazol (please reach out to us, we can supply this), or Trizol. Please do not use >700 ul of Qiazol.

MagMAX mirVana

For high throughput projects, we recommend the 96-well, plate-based MagMAX mirVana Total RNA Isolation Kit from Thermo Fisher.

https://www.thermofisher.com/order/catalog/product/A27828

This kit purifies RNA from 10-30 mg of tissue or 1 × 106 cells.

Samples should be provided to the Genome Core frozen in the MagMax Processing Plate in MagMax Lysis Binding Mix (please reach out to us, we can supply these), according to your project’s unique specifications. We will work with you to make sure that you have all of the information and supplies.