Latest revision as of 17:08, 10 July 2024

Library prep

RNA

Watchmaker for mRNA

For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.

Watchmaker for Ribodepletion

Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis.

NEBnext small RNA

For microRNA and other small RNA. 100-1000ng total RNA.

Clonetech SMART-Seq v4 Ultra-low

For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.

IDT/xGen for mRNA (Supported Only For Legacy Projects)

For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.

KAPA Hyperprep mRNA (Supported Only For Legacy Projects)

For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.

KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)

Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)

Clonetech SMARTer PICO (Supported Only For Legacy Projects)

For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA.

DNA

Nextera gDNA

“Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.