Difference between revisions of "Pricing"

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= Library Prep =
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= RNA Library Prep =
 
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{{PricingRow|Swift ChIP DNA (ChIP-Seq)|Sample|$200|Optimized for low input ChIP}}
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{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12|Sample|$200|}}
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{{PricingRow|Illumina DNA (Genomic)|Sample|$200|Genomic DNA >100ng}}
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{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+|Sample|$150|}}
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{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|Sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
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{{PricingRow|KAPA Hyper Prep Ribo Zero, samples 1-12|Sample|$250|}}
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{{PricingRow|KAPA Hyper Prep - Stranded (RNA-Seq)|Sample|$200|}}
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{{PricingRow|KAPA Hyper Prep Ribo Zero, samples 13+|Sample|$200|}}
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{{PricingRow|Swift - Stranded (RNA-Seq)|Sample|$200|}}
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{{PricingRow|Agilent SureSelect All Exon|Sample|$650|}}
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{{PricingRow|KAPA Ribo Erase (RNA-Seq)|Sample|$250|}}
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{{PricingLastRow|NEBNext Small RNA|Sample|$200|}}
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{{PricingRow|Clontech UltraLow RNA (RNA-Seq)|Sample|$300|For low input RNA down to single cell}}
 
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{{PricingRow|Clontech Stranded Total RNA (RNA-Seq)|Sample|$250|-}}
 
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{{PricingRow|NEBNext Small RNA (microRNA)|Sample|$200|-}}
 
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{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 3’ Solution Library prep (RNA-Seq)]|Sample|$2,362|Sorting and library prep for up to 10,000 cells}}
 
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{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 5’ Solution Library prep (RNA-Seq)]|Sample|$2,362|Sorting and library prep for up to 10,000 cells}}
 
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{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/SingleCell SMART-seq v2 Library prep (RNA-Seq)]|96-well Plate|$2,500|}}
 
  
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Revision as of 13:07, 19 January 2023

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Illumina Sequencing

There are three options for Illumina sequencing. NovaSeq and MiSeq.

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

NovaSeq (Single lane submissions accepted for standard read lengths)

Item Read Length Unit Price Per Unit Notes
NovaSeqSP 100 Single End or 50x50 Paired-End 1 lane $1,712.50 ~650–800 million reads per flowcell
~325-400 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $2,075
300 Single End or 150x150 Paired-End $2,425
500 Single End or 250x250 Paired-End $3,250
NovaSeqS1 100 Single End or 50x50 Paired-End 1 lane $2,700 ~1.3–1.6 billion reads per flowcell
~650–800 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $3,450
300 Single End or 150x150 Paired-End $3,762.50
NovaSeqS2 100 Single End or 50x50 Paired-End 1 lane $4,812.50 ~3.3–4.1 billion reads per flowcell
~1.65-2.05 billion reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $6,000
300 Single End or 150x150 Paired-End $6,375
NovaSeqS4 35 Single End 1 lane $3,600 ~8-10 billion reads per flowcell
~2-2.5 billion reads per lane
(x2 for Paired-End)
300 Single End or 150x150 Paired-End $5,000



MiSeq -- 20 million reads

Item Unit Price Per Unit Notes
MiSeq v3 150 Single End or 75x75 Paired End Flowcell $1,300


RNA Library Prep

Item Unit Price Per Unit Notes
IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12 Sample $200
IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+ Sample $150
KAPA Hyper Prep Ribo Zero, samples 1-12 Sample $250
KAPA Hyper Prep Ribo Zero, samples 13+ Sample $200
Agilent SureSelect All Exon Sample $650
NEBNext Small RNA Sample $200


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer Sample $15 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run



nCounter Analysis System (NanoString)

Item Unit Price Per Unit Notes
Reagents Cartridge ---- Reagents and Codesets Purchase From Nanostring
Equipment Use Cartridge $250 12 samples per cartridge
Labor-Hyb Cartridge $150 -
Labor-miRNA Sample $25 Addon for miRNA protocol only


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - QuantStudio 6

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7 -
Equipment Time (2 hour time block) Plate $30 Sign-up Required


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