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Revision as of 14:52, 12 January 2015
Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.
Contents
Illumina Sequencing
There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.
The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.
Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.
HiSeq (Std)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| HiSeq - 40 bp Single Read | Lane | $1,085 | avg 120-130 million reads |
| HiSeq - 40x40 Paired End | Lane | $1,625 | avg 120-130 million paired end reads |
| HiSeq - 75x75 Paired End | Lane | $2,155 | avg 120-130 million paired end reads |
| HiSeq - 100x100 Paired End | Lane | $2,525 | avg 120-130 million paired end reads |
| HiSeq - Additional 40 bases | Lane | $300 | Add to above base prices for longer reads |
HiSeq (Rapid)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| HiSeq2500 - 50 bp Single Read | Sample | $1,600 | avg 100-120 million reads |
| HiSeq2500 - 40x40 Paired End | Sample | $2,400 | avg 100-120 million paired end reads |
| HiSeq2500 - 100x100 Paired End | Sample | $3,100 | avg 100-120 million paired end reads |
| HiSeq2500 - 250x250 Paired End | Sample | $5,300 | avg 100-120 million paired end reads |
| HiSeq2500 - Additional 50 bases | Sample | $500 | Add to above base prices for longer reads up to 250x250 |
| HiSeq2500 - Add DUAL mode | Sample | $440 | For running different samples in each lane |
Library Prep
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| DNA-Seq Library prep - Basic | Sample | $200 | Automated protocol suitable for genomic DNA |
| DNA-Seq Library prep - Premium | Sample | $275 | Manual Protocol ideal for ChIP-seq and other low-input applications |
| RNA-Seq Library prep - Basic | Sample | $200 | Automated protocol, requires minimum 100ng total RNA, single stranded |
| RNA-Seq Library prep - Premium | Sample | $275 | Manual Protocol, uses Illumina "TruSeq" kits |
| RNA-Seq add RiboZero | Sample | $165 | Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost) |
| Sample Multiplexing | Sample | $10 | Mixing samples for multiplex run |
| Quality Control Only | Sample | $40 | Bioanalyzer and qPCR |
Quality Control
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Bioanalyzer | Sample | $15 | Sizing and purity analysis |
| qPCR | Sample | $25 | Quantification |
| Sample Multiplexing | Sample | $10 | Mixing samples for multiplex run |
Microarray Services
Prices for sample processing do not include cost of arrays
Affymetrix Array Processing
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Full Service Expression Analysis - 3'IVT or WT | Sample | $325 | Not including cost of Array |
| Contact GTC or Affymetrix for array prices | Array | $150-$400 | Array only |
Agilent Array Processing
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Full Service Expression Analysis (Single Channel) | Sample | $325 | Not including cost of array |
| Full Service Expression Analysis (Two Samples - Dual Channel) | Two Samples | $525 | Not including cost of array |
| Contact GTC or Agilent for Array pricing | Array | $$ |
nCounter Analysis System (NanoString)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Equipment Use | Cartridge | $190 | 12 samples per cartridge |
| Reagents | Sample | $23.00* | *Does NOT include CodeSet |
| Labor | Cartridge | $105.00 | - |
Bioanalyzer - RNA, DNA and Protein analysis
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| RNA or DNA | Sample | $15 | Pico, Nano, DNA or hsDNA |
Quantitative PCR - ABI 7900
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| SYBR green / Taq Man Master mix | uL | 7 cents | - |
| Optical Plate | Plate | $7.00 | - |
| Equipment Time (2 hour time block) | Plate | $30.00 | Sign-up Required |
