Difference between revisions of "Pricing"

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{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$1,600|avg ~160-200 million reads}}
 
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$1,600|avg ~160-200 million reads}}
 
{{PricingRow|HiSeqRapid - 40 bp Single Read|1 lane|$1,085|avg ~80-100 million reads}}
 
{{PricingRow|HiSeqRapid - 40 bp Single Read|1 lane|$1,085|avg ~80-100 million reads}}
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{{PricingRow|HiSeqRapid - 40x40 Paired End|full flowcell (2 lanes)|$2,400|avg ~160-200 million paired end reads}}
+
{{PricingRow|HiSeqRapid - 50x50 Paired End|full flowcell (2 lanes)|$2,400|avg ~160-200 million paired end reads}}
−
{{PricingRow|HiSeqRapid - 40x40 Paired End|1 lane|$1,420|avg ~80-100 million paired end reads}}
+
{{PricingRow|HiSeqRapid - 50x50 Paired End|1 lane|$1,420|avg ~80-100 million paired end reads}}
 
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$3,100|avg ~160-200 million paired end reads}}
 
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$3,100|avg ~160-200 million paired end reads}}
 
{{PricingRow|HiSeqRapid - 100x100 Paired End|1 lane|$1,770|avg ~80-100 million paired end reads}}
 
{{PricingRow|HiSeqRapid - 100x100 Paired End|1 lane|$1,770|avg ~80-100 million paired end reads}}

Revision as of 13:12, 20 July 2015

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Illumina Sequencing

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to 10 days in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

HiSeq (Std)

Item Unit Price Per Unit Notes
HiSeq - 40 bp Single Read Lane $1,085 avg 120-150 million reads
HiSeq - 40x40 Paired End Lane $1,625 avg 120-150 million paired end reads
HiSeq - 75x75 Paired End Lane $2,155 avg 120-150 million paired end reads
HiSeq - 100x100 Paired End Lane $2,525 avg 120-150 million paired end reads
HiSeq - Additional 40 bases Lane $300 Add to above base prices for longer reads


HiSeq (Rapid)

Item Unit Price Per Unit Notes
HiSeqRapid - 50 bp Single Read full flowcell (2 lanes) $1,600 avg ~160-200 million reads
HiSeqRapid - 40 bp Single Read 1 lane $1,085 avg ~80-100 million reads
HiSeqRapid - 50x50 Paired End full flowcell (2 lanes) $2,400 avg ~160-200 million paired end reads
HiSeqRapid - 50x50 Paired End 1 lane $1,420 avg ~80-100 million paired end reads
HiSeqRapid - 100x100 Paired End full flowcell (2 lanes) $3,100 avg ~160-200 million paired end reads
HiSeqRapid - 100x100 Paired End 1 lane $1,770 avg ~80-100 million paired end reads
HiSeqRapid - 250x250 Paired End full flowcell (2 lanes) $5,300 avg ~160-200 million paired end reads
HiSeqRapid - Add DUAL mode full flowcell (2 lanes) $440 ONLY required if running different samples in each lane
HiSeqRapid - Additional 50 bases full flowcell (2 lanes) $500 Add to above base prices for longer reads up to 250x250


Library Prep

Item Unit Price Per Unit Notes
TruSeq ChIP DNA Sample $200 Optimized for low DNA input
TruSeq Nano gDNA Sample $200 Genomic DNA >100ng
TruSeq Stranded mRNA Sample $200 Basic RNA Expression Analysis
TruSeq Stranded RiboZero Sample $250 Replaces polyA selection with a ribozomal RNA depletion
Clontech UltraLow RNA Sample $400 For low input RNA down to single cell
Nextera genomic DNA Sample $200 "Tagmentation" prep from intact gDNA (50ng)
Sample Multiplexing Sample $10 Mixing samples for multiplex run
Quality Control Only Sample $40 Bioanalyzer and qPCR


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer Sample $15 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run


Microarray Services

Prices for sample processing do not include cost of arrays

Affymetrix Array Processing

Item Unit Price Per Unit Notes
Full Service Expression Analysis - 3'IVT or WT Sample $325 Not including cost of Array
Contact GTC or Affymetrix for array prices Array $150-$400 Array only



Agilent Array Processing

Item Unit Price Per Unit Notes
Full Service Expression Analysis (Single Channel) Sample $325 Not including cost of array
Full Service Expression Analysis (Two Samples - Dual Channel) Two Samples $525 Not including cost of array
Contact GTC or Agilent for Array pricing Array $$



nCounter Analysis System (NanoString)

Item Unit Price Per Unit Notes
Equipment Use Cartridge $190 12 samples per cartridge
Reagents Sample $23.00* *Does NOT include CodeSet
Labor Cartridge $105.00 -


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - ABI 7900

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7.00 -
Equipment Time (2 hour time block) Plate $30.00 Sign-up Required


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