Difference between revisions of "Template:SeqSubmissionGuide"
From Genome Technology Core (GTC) wiki - Sequencing and Microarray
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=SEQUENCING SUBMISSION GUIDELINES= | =SEQUENCING SUBMISSION GUIDELINES= | ||
| − | + | ==STEP 1: REGISTRATION (FOR NEW CUSTOMERS ONLY)== | |
| − | + | * INTERNAL CUSTOMERS: Do NOT require to register in the system | |
| − | * | + | * EXTERNAL CUSTOMERS: Have your lab administrator register in the Whitehead Cores Database - https://cores.wi.mit.edu/. |
| − | |||
All external customers please obtain a purchase order number prior to your submission. If you need a quote, please email us at wibr-genome@wi.mit.edu or call us at 617-258-8803. Blanket purchase orders are preferred, if you plan to submit multiple projects over time. | All external customers please obtain a purchase order number prior to your submission. If you need a quote, please email us at wibr-genome@wi.mit.edu or call us at 617-258-8803. Blanket purchase orders are preferred, if you plan to submit multiple projects over time. | ||
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===SUBMISSION OF SAMPLES FOR LIBRARY PREPARATION=== | ===SUBMISSION OF SAMPLES FOR LIBRARY PREPARATION=== | ||
| − | Samples should be in a buffer that does not contain trace amounts of phenol or EDTA. We will save and return any unused input material after libraries are successfully prepared | + | Samples should be in a buffer that does not contain trace amounts of phenol or EDTA. We will save and return any unused input material after libraries are successfully prepared. |
Below are our input requirements for Library Prep Service | Below are our input requirements for Library Prep Service | ||
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| 200 to 400 | | 200 to 400 | ||
| 500 to 1000 | | 500 to 1000 | ||
| − | | | + | | 10-50 |
|- | |- | ||
! scope="row"| ChIP | ! scope="row"| ChIP | ||
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| Variable (specify) | | Variable (specify) | ||
| 10 to 50 | | 10 to 50 | ||
| − | | | + | | 10-40 |
|- | |- | ||
! scope="row"| RNA (total) | ! scope="row"| RNA (total) | ||
| Line 44: | Line 43: | ||
| NA | | NA | ||
| 100 to 1000 | | 100 to 1000 | ||
| − | | | + | | 10-50 |
|} | |} | ||
<nowiki>*</nowiki>Unless special arrangements are made with us in advance, we will assume that your genomic DNA sample has been fragmented, by sonication or otherwise, prior to submission. | <nowiki>*</nowiki>Unless special arrangements are made with us in advance, we will assume that your genomic DNA sample has been fragmented, by sonication or otherwise, prior to submission. | ||
| − | <nowiki>**</nowiki>We can work with less if necessary, but it is best if you elute your DNA in 50 ul or less if you believe we may need to use all of it. | + | <nowiki>**</nowiki>We can work with less if necessary, but it is best if you elute your DNA in 50 ul or less if you believe we may need to use all of it. Please give us at least 10uL of sample to ensure we have enough for QC and prep. |
| − | |||
===SUBMISSION OF PRE-PREPARED LIBRARIES=== | ===SUBMISSION OF PRE-PREPARED LIBRARIES=== | ||
| − | We typically need 10-20 ul of each library at 5 ng/ul or greater. If your library is less concentrated than 5 ng/ul, we’ll quantify it to make sure we have enough to sequence | + | We typically need 10-20 ul of each library at 5 ng/ul or greater. If your library is less concentrated than 5 ng/ul, we’ll quantify it to make sure we have enough to sequence. |
==STEP 3: ONLINE SUBMISSION OF SEQUENCING LIBRARIES== | ==STEP 3: ONLINE SUBMISSION OF SEQUENCING LIBRARIES== | ||
| − | *Please complete the online submission form (Available Here). Questions or problems related to the submission | + | *Please complete the online submission form ([http://jura.wi.mit.edu/genomecorewiki/index.php/Forms Link To Form Available Here]). Questions or problems related to the submission should be directed to Sumeet Gupta at sgupta@wi.mit.edu |
*Every physical sample tube should have its own Genome Tech Core ID (GTC ID) number (automatically generated and emailed to you immediately after submission of the online form), even if you plan to multiplex them. | *Every physical sample tube should have its own Genome Tech Core ID (GTC ID) number (automatically generated and emailed to you immediately after submission of the online form), even if you plan to multiplex them. | ||
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Whitehead Institute Genome Technology Core <br> | Whitehead Institute Genome Technology Core <br> | ||
| − | + | 455 Main Street, Room 325<br> | |
Cambridge, MA 02142 | Cambridge, MA 02142 | ||
Please do not ship without first contacting someone in the lab, to make sure that we are here to receive your package. Do not rely on voicemail. Please wait until you hear back from someone. | Please do not ship without first contacting someone in the lab, to make sure that we are here to receive your package. Do not rely on voicemail. Please wait until you hear back from someone. | ||
Latest revision as of 11:20, 30 September 2019
Contents
SEQUENCING SUBMISSION GUIDELINES
STEP 1: REGISTRATION (FOR NEW CUSTOMERS ONLY)
- INTERNAL CUSTOMERS: Do NOT require to register in the system
- EXTERNAL CUSTOMERS: Have your lab administrator register in the Whitehead Cores Database - https://cores.wi.mit.edu/.
All external customers please obtain a purchase order number prior to your submission. If you need a quote, please email us at wibr-genome@wi.mit.edu or call us at 617-258-8803. Blanket purchase orders are preferred, if you plan to submit multiple projects over time.
STEP 2: PREPARATION OF SAMPLES
SUBMISSION OF SAMPLES FOR LIBRARY PREPARATION
Samples should be in a buffer that does not contain trace amounts of phenol or EDTA. We will save and return any unused input material after libraries are successfully prepared.
Below are our input requirements for Library Prep Service
| Sample Type | Sonication/Fragmentation* | Fragment Size (bp) | Input requirements (ng)** | Volume (ul) |
|---|---|---|---|---|
| Genomic | Required | 200 to 400 | 500 to 1000 | 10-50 |
| ChIP | Required | Variable (specify) | 10 to 50 | 10-40 |
| RNA (total) | NA | NA | 100 to 1000 | 10-50 |
*Unless special arrangements are made with us in advance, we will assume that your genomic DNA sample has been fragmented, by sonication or otherwise, prior to submission.
**We can work with less if necessary, but it is best if you elute your DNA in 50 ul or less if you believe we may need to use all of it. Please give us at least 10uL of sample to ensure we have enough for QC and prep.
SUBMISSION OF PRE-PREPARED LIBRARIES
We typically need 10-20 ul of each library at 5 ng/ul or greater. If your library is less concentrated than 5 ng/ul, we’ll quantify it to make sure we have enough to sequence.
STEP 3: ONLINE SUBMISSION OF SEQUENCING LIBRARIES
- Please complete the online submission form (Link To Form Available Here). Questions or problems related to the submission should be directed to Sumeet Gupta at sgupta@wi.mit.edu
- Every physical sample tube should have its own Genome Tech Core ID (GTC ID) number (automatically generated and emailed to you immediately after submission of the online form), even if you plan to multiplex them.
- Please write your name GTC ID numbers on the tubes. We prefer 1.5 ml tubes.
STEP 4: DROP-OFF OR SHIPPING
When you are ready, you may hand deliver or ship samples them on dry ice to:
Whitehead Institute Genome Technology Core
455 Main Street, Room 325
Cambridge, MA 02142
Please do not ship without first contacting someone in the lab, to make sure that we are here to receive your package. Do not rely on voicemail. Please wait until you hear back from someone.
