Difference between revisions of "Pricing"

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(→‎Illumina MiSeq i100 plus (Internal WI Pricing) - WALK UP ONLY PRICING)
 
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−
Prices quoted are for Whitehead Institute/MIT only.  In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.
+
Prices quoted are for Whitehead Institute/MIT only.  In order to cover additional costs associated with providing outside service, external non-profit/academic labs would only pay a 15% surcharge. If the data being generated will be in direct collaboration with a Whitehead researcher, we are happy to extend the internal pricing without any surcharge. Non-academic/commerical entities should add 30% to the list price.
  
  
−
= Illumina Sequencing =
+
= Sequencing =
 
 
−
There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".
+
There are three options for Illumina sequencing. NovaSeq and MiSeq.
  
 
Prices include basic data processing including base calls, quality scores and alignment (if requested).  
 
Prices include basic data processing including base calls, quality scores and alignment (if requested).  
  
−
Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.
+
Please visit the [http://gtc.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.
  
−
=== NovaSeq ===
+
== Short Read (Single lane submissions accepted for standard read lengths) ==
 +
 
 +
=== Illumina NovaSeq ===
 +
 
 +
{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
 +
|-
 +
| Item || Read Length || Unit || Price Per Unit || Notes
 +
|-
 +
|rowspan="4"|NovaSeqSP
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="4"| 1 lane
 +
|$1,850
 +
|rowspan="4"|~650–800 million reads per flowcell <br>~325-400 million reads per lane <br>(x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$2,245
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$2,620
 +
|-
 +
| 500 Single End or 250x250 Paired-End
 +
|$3,510
 +
|-
 +
|rowspan="3"|NovaSeqS1
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="3"|1 lane
 +
|$2,920
 +
|rowspan="3"|~1.3–1.6 billion reads per flowcell <br> ~650–800 million reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$3,730
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$4,065
 +
|-
 +
|rowspan="3"|NovaSeqS2
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="3"|1 lane
 +
|$5,200
 +
|rowspan="3"|~3.3–4.1 billion reads per flowcell <br> ~1.65-2.05 billion reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$6,480
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$6,750
 +
|-
 +
|rowspan="2"|NovaSeqS4
 +
| 50 Single End
 +
|rowspan="2"|1 lane
 +
|$3,780
 +
|rowspan="2"|~8-10 billion reads per flowcell <br> ~2-2.5 billion reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$4,750
 +
|-
 +
|}
 +
 
 +
 
 +
<br>
 +
 
 +
=== Element AVITI ===
 +
{{PricingHeader}}
 +
{{PricingRow|150 Single End or 75x75 Paired End|Lane|$1,000| 400 million reads}}
 +
{{PricingRow|300 Single End or 150x150 Paired End|Lane|$1,375| 400 million reads}}
 +
{{PricingLastRow|300x300 Paired End|Flowcell|$3,500| 300 million reads}}
 +
 
 +
 
 +
<br>
 +
 
 +
=== Illumina MiSeq i100 plus (Internal WI Pricing) - WALK UP ONLY PRICING ===
 +
If a library pool is provided to GTC (instead of setting up the run yourself), please add $300 to the cost below which includes QC (qubit, FA and qPCR), sequencing, basic data processing and data storage.
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|NovaSeqSP - 100bp Single Read OR 50x50bp Paired End|full flowcell (2 lanes)|$3,533|Around 325 Million or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Series 5M Reagent Kit (300 cycles)|Flowcell|$500| 5 million reads}}
−
{{PricingRow|NovaSeqSP - 300bp Single Read OR 150x150bp Paired End|full flowcell (2 lanes)|$5,545|Around 325 Million or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Series 5M Reagent Kit (600 cycles)|Flowcell|$700| 5 million reads}}
−
{{PricingRow|NovaSeqSP - 500bp Single Read OR 250x250bp Paired End|full flowcell (2 lanes)|$7,613|Around 325 Million or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Series 25M Reagent Kit (100 cycles)|Flowcell|$600| 25 million reads}}
−
{{PricingRow|NovaSeqS1 - 100bp Single Read OR 50x50bp Paired End|full flowcell (2 lanes)|$5,565|Around 650 Million or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Series 25M Reagent Kit (300 cycles)|Flowcell|$950| 25 million reads}}
−
{{PricingRow|NovaSeqS1 - 200bp Single Read OR 100x100bp Paired End|full flowcell (2 lanes)|$7,245|Around 650 Million or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Series 25M Reagent Kit (600 cycles)|Flowcell|$1,100| 25 million reads}}
−
{{PricingRow|NovaSeqS1 - 300bp Single Read OR 150x150bp Paired End|full flowcell (2 lanes)|$8,610|Around 650 Million or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Series 25M Reagent Kit (1000 cycles)|Flowcell|$1,650| 25 million reads}}
−
{{PricingRow|NovaSeqS2 - 100bp Single Read OR 50x50bp Paired End|full flowcell (2 lanes)|$11,445|Around 1.6 Billion or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Plus 50M Reagent Kit (100 cycles)|Flowcell|$800| 50 million reads}}
−
{{PricingRow|NovaSeqS2 - 200bp Single Read OR 100x100bp Paired End|full flowcell (2 lanes)|$15,435|Around 1.6 Billion or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Plus 50M Reagent Kit (300 cycles)|Flowcell|$1,100| 50 million reads}}
−
{{PricingRow|NovaSeqS2 - 300bp Single Read OR 150x150bp Paired End|full flowcell (2 lanes)|$18,060|Around 1.6 Billion or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Plus 50M Reagent Kit (600 cycles)|Flowcell|$1,400| 50 million reads}}
−
{{PricingRow|NovaSeqS4 - 300bp Single Read OR 150x150bp Paired End|full flowcell (4 lanes)|Contact Us|Around 2 Billion or more Reads Per Lane}}
+
{{PricingRow|MiSeq i100 Plus 100M Reagent Kit (100 cycles)|Flowcell|$1,000| 100 million reads}}
−
{{PricingLastRow||||}}
+
{{PricingLastRow|MiSeq i100 Plus 100M Reagent Kit (300 cycles)|Flowcell|$1,400| 100 million reads}}
  
 
<br>
 
<br>
  
−
=== HiSeq (Std a.k.a. "High Output" mode) -- At least 150-200 million reads per lane ===
+
== Long Read ==
−
Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
+
 
 +
=== Nanopore (P2 Solo/promethION) ===
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|HiSeq - 50 bp Single Read|Lane|$1,200|}}
+
{{PricingLastRow|Nanopore|Flowcell|$1200| Upto 50 to 70GB data per flow cell}}
−
{{PricingRow|HiSeq - 28x40 10X|Lane|$1,500|ONLY FOR 10X LIBRARIES}}
 
−
{{PricingRow|HiSeq - 60x60 Paired End|Lane|$1,750|}}
 
−
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,250|}}
 
−
{{PricingRow|HiSeq - Additional 70 bases|Lane|$400|Add to above base prices for longer reads}}
 
  
−
{{PricingLastRow||||}}
 
 
<br>
 
<br>
  
−
=== HiSeq (Rapid) -- At least 100 to 150 million reads per lane (Two lanes per Flow Cell) ===
+
=== Pacbio ===
−
In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250, and complete more quickly. For example - a 100x100 run in Rapid mode completes in 2-3 days vs up to 7 days in Standard mode.
 
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$2,300|}}
+
{{PricingLastRow|Pacbio SMRTCell 25 Million|Run|$2250|Around 6 million reads, outsourcing to sister facilities to enable access to the REVIO}}
−
{{PricingRow|HiSeqRapid - 28x40 10X|full flowcell (2 lanes)|$2,800|ONLY FOR 10X LIBRARIES}}
 
−
{{PricingRow|HiSeqRapid - 60x60 Paired End|full flowcell (2 lanes)|$3,400|}}
 
−
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$4,350|}}
 
−
{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$7,200|}}
 
−
{{PricingRow| | | | }}
 
−
{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$460| ONLY required if running different samples in each lane}}
 
−
{{PricingRow| | | | }}
 
−
{{PricingRow|HiSeqRapid - Additional 70 bases|full flowcell (2 lanes)|$560|Add to above base prices for longer reads up to 250x250}}
 
−
{{PricingLastRow||||}}
 
  
 
<br>
 
<br>
  
−
=== Library Prep ===
+
= RNA Library Prep =
 +
=== Standard (Manual) ===
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|Swift ChIP DNA (ChIP-Seq)|Sample|$200|Optimized for low input ChIP}}
+
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples 1-12|Sample|$200|}}
−
{{PricingRow|TruSeq Nano gDNA (Genomic)|Sample|$200|Genomic DNA >100ng}}
+
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples 13-24|Sample|$185|}}
−
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|Sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
+
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples 25-48|Sample|$135|}}
−
{{PricingRow|KAPA Hyper Prep - Stranded (RNA-Seq)|Sample|$200|}}
+
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples >48|Sample|$100|}}
−
{{PricingRow|KAPA Ribo Erase (RNA-Seq)|Sample|$250|}}
+
{{PricingRow|Watchmaker mRNA, samples 1-12|Sample|$150|}}
−
{{PricingRow|Clontech UltraLow RNA (RNA-Seq)|Sample|$300|For low input RNA down to single cell}}
+
{{PricingRow|Watchmaker mRNA, samples 13-24|Sample|$135|}}
−
{{PricingRow|Clontech Stranded Total RNA Pico (RNA-Seq)|Sample|$250|-}}
+
{{PricingRow|Watchmaker mRNA, samples 25-48|Sample|$85|}}
−
{{PricingRow|NEBNext Small RNA (microRNA)|Sample|$200|-}}
+
{{PricingRow|Watchmaker mRNA, samples >48|Sample|$50|}}
−
{{PricingRow|[http://jura.wi.mit.edu/genomecorewiki/index.php/SingleCell 10X Single Cell 3’ Solution Library prep (RNA-Seq)]|Sample|$2,000|Sorting and library prep for up to 10,000 cells}}
+
{{PricingRow|Agilent SureSelect All Exon|Sample|$650|}}
−
{{PricingLastRow|[http://jura.wi.mit.edu/genomecorewiki/index.php/SingleCell SMART-seq v2 Library prep (RNA-Seq)]|96-well Plate|$2,500|}}
+
{{PricingLastRow|NEBNext Small RNA|Sample|$200|}}
 +
 
 +
<br>
  
−
{| style="background:white; color:black;" align="right"
+
 
−
| [[LibraryPrep|Click here for more info about Library Prep options]]
+
=== Low Input (Manual) ===
 +
{{PricingHeader}}
 +
{{PricingLastRow|Clontech SMARTer UltraLow RNA w/ NexteraXT|Sample|$300|}}
 +
 
 +
<br>
 +
 
 +
 
 +
=== Highthroughput (96 well Plate Based) ===
 +
 
 +
{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
 +
|-
 +
| Item || Unit || Price Per Unit || Notes
 +
|-
 +
|rowspan="2"|HT mRNA library prep (Watchmaker)
 +
| 48 Samples
 +
|$2000
 +
|rowspan="2" width=50%| Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.
 +
Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.
 +
 
 +
Libraries are pooled by FA quantification, expect variation in read balancing. 
 +
|-
 +
| 96 samples
 +
|$4000
 +
|-
 +
|rowspan="4"| Alithea Genomics DRUG-Seq (3')
 +
| 24 Samples
 +
|$1500
 +
|rowspan="4" width=50%| 3' biased RNA-Seq - One Read per RNA molecule
 +
|-
 +
| 48 samples
 +
|$2000
 +
|-
 +
|-
 +
| 72 samples
 +
|$2500
 +
|-
 +
|-
 +
| 96 samples
 +
|$3000
 +
|-
 +
|-
 +
|rowspan="4"| Alithea Genomics DRUG-Seq (Full Length)
 +
| 24 Samples
 +
|$1800
 +
|rowspan="4" width=50%| RNA-Seq - Reads across the whole RNA molecule
 +
|-
 +
| 48 samples
 +
|$2300
 +
|-
 +
|-
 +
| 72 samples
 +
|$2800
 +
|-
 +
|-
 +
| 96 samples
 +
|$3300
 +
|-
 +
|rowspan="1"| Alithea Genomics FLASH-Seq
 +
| 96 Samples
 +
|$4500
 +
|rowspan="1" width=50%| Single cell plate-based RNA-Seq
 +
|-
 
|}
 
|}
 +
 
<br>
 
<br>
  
−
=== Quality Control ===
+
= Single Cell (10X and others) =
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
+
{{PricingRow|10X Single Cell 3’ GEX (Includes OCM if requested)|sample|$2560|}}
−
{{PricingRow|qPCR|Sample|$25|Quantification}}
+
{{PricingRow|10X Single Cell 5’ GEX|sample|$2560|}}
−
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
+
{{PricingRow|10X VDJ Add-on|sample|$230|Can be added onto the 10X 5' GEX Only}}
 +
{{PricingRow|10X CRISPR Add-on|sample|$280|Can be added onto the 10X 5' GEX Only}}
 +
{{PricingRow|10X Antibody/CellPlex/MultiSeq Add-on|sample|$140|}}
 +
{{PricingRow|10X Multiome Assay (ATAC + 3')|sample|$4200|}}
 +
{{PricingRow|10X Flex (Fixed Cell)|sample|$2800|Human and Mouse Only}}
 +
{{PricingLastRow|Alithea Single Cell 96 well plates (FLASH-Seq)|96 well plate|$4500|1 cell per well}}
 +
 
 
<br>
 
<br>
  
 +
= DNA Library Prep =
 +
{{PricingHeader}}
 +
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
 +
{{PricingLastRow|Illumina DNA Prep (Genomic)|sample|$200|Genomic DNA >100ng}}
  
−
= nCounter Analysis System (NanoString) =
+
<br>
 +
 
 +
= RNA Isolation =
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|Reagents|Cartridge|----|Reagents and Codesets Purchase From Nanostring}}
+
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation 1-12 samples]|sample|$85|}}
−
{{PricingRow|Equipment Use|Cartridge|$250|12 samples per cartridge}}
+
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation 12-24 samples]|sample|$55|}}
−
{{PricingRow|Labor-Hyb|Cartridge|$150|-}}
+
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation 25-48 samples]|sample|$35|}}
−
{{PricingLastRow|Labor-miRNA|Sample|$25|Addon for miRNA protocol only}}
+
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation >48 samples]|sample|$25|}}
 +
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA - MagMax mirVana RNA Isolation]|sample|$30|}}
 +
{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA - Direct-zol™-96 MagBead RNA Isolation]|sample|$30|}}
  
 
<br>
 
<br>
 +
 +
= Quality Control =
 +
{{PricingHeader}}
 +
{{PricingRow|Bioanalyzer/Fragment Analyzer|1 to 20 Samples|$15|Sizing and purity analysis}}
 +
{{PricingRow|Fragment Analyzer (half 96 well plate)|20 to 48 Samples|$300|Sizing and purity analysis}}
 +
{{PricingRow|Fragment Analyzer (Full 96 well plate)|49+ Samples|$500|Sizing and purity analysis}}
 +
{{PricingRow|qPCR|Sample|$25|Quantification}}
 +
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 +
 +
<br>
 +
 
= Bioanalyzer - RNA, DNA and Protein analysis =
 
= Bioanalyzer - RNA, DNA and Protein analysis =
 
{{PricingHeader}}
 
{{PricingHeader}}
 
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}
 
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}
 +
 +
<br>
 +
 +
= Sizing/Cleanup =
 +
{{PricingHeader}}
 +
{{PricingRow|Bead Sizing (Pippin) Up to 12 samples|Sample|$25|}}
 +
{{PricingRow|Bead Sizing (Pippin) Over 12 samples|Sample|$15|}}
 +
{{PricingRow|Gel Sizing (Pippin) Up to 12 samples|Sample|$50|}}
 +
{{PricingLastRow|Gel Sizing (Pippin) Over 12 samples|Sample|$25|}}
  
 
<br>
 
<br>
Line 98: Line 254:
 
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
 
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
 
{{PricingRow|Optical Plate|Plate|$7|-}}
 
{{PricingRow|Optical Plate|Plate|$7|-}}
−
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30|Sign-up Required}}
+
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$50|Sign-up Required}}
  
 
<br>
 
<br>
 +
 +
= Digital PCR =
 +
{{PricingHeader}}
 +
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$50|Sign-up Required}}

Latest revision as of 13:46, 6 February 2026

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, external non-profit/academic labs would only pay a 15% surcharge. If the data being generated will be in direct collaboration with a Whitehead researcher, we are happy to extend the internal pricing without any surcharge. Non-academic/commerical entities should add 30% to the list price.


Sequencing

There are three options for Illumina sequencing. NovaSeq and MiSeq.

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

Short Read (Single lane submissions accepted for standard read lengths)

Illumina NovaSeq

Item Read Length Unit Price Per Unit Notes
NovaSeqSP 100 Single End or 50x50 Paired-End 1 lane $1,850 ~650–800 million reads per flowcell
~325-400 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $2,245
300 Single End or 150x150 Paired-End $2,620
500 Single End or 250x250 Paired-End $3,510
NovaSeqS1 100 Single End or 50x50 Paired-End 1 lane $2,920 ~1.3–1.6 billion reads per flowcell
~650–800 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $3,730
300 Single End or 150x150 Paired-End $4,065
NovaSeqS2 100 Single End or 50x50 Paired-End 1 lane $5,200 ~3.3–4.1 billion reads per flowcell
~1.65-2.05 billion reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $6,480
300 Single End or 150x150 Paired-End $6,750
NovaSeqS4 50 Single End 1 lane $3,780 ~8-10 billion reads per flowcell
~2-2.5 billion reads per lane
(x2 for Paired-End)
300 Single End or 150x150 Paired-End $4,750



Element AVITI

Item Unit Price Per Unit Notes
150 Single End or 75x75 Paired End Lane $1,000 400 million reads
300 Single End or 150x150 Paired End Lane $1,375 400 million reads
300x300 Paired End Flowcell $3,500 300 million reads



Illumina MiSeq i100 plus (Internal WI Pricing) - WALK UP ONLY PRICING

If a library pool is provided to GTC (instead of setting up the run yourself), please add $300 to the cost below which includes QC (qubit, FA and qPCR), sequencing, basic data processing and data storage.

Item Unit Price Per Unit Notes
MiSeq i100 Series 5M Reagent Kit (300 cycles) Flowcell $500 5 million reads
MiSeq i100 Series 5M Reagent Kit (600 cycles) Flowcell $700 5 million reads
MiSeq i100 Series 25M Reagent Kit (100 cycles) Flowcell $600 25 million reads
MiSeq i100 Series 25M Reagent Kit (300 cycles) Flowcell $950 25 million reads
MiSeq i100 Series 25M Reagent Kit (600 cycles) Flowcell $1,100 25 million reads
MiSeq i100 Series 25M Reagent Kit (1000 cycles) Flowcell $1,650 25 million reads
MiSeq i100 Plus 50M Reagent Kit (100 cycles) Flowcell $800 50 million reads
MiSeq i100 Plus 50M Reagent Kit (300 cycles) Flowcell $1,100 50 million reads
MiSeq i100 Plus 50M Reagent Kit (600 cycles) Flowcell $1,400 50 million reads
MiSeq i100 Plus 100M Reagent Kit (100 cycles) Flowcell $1,000 100 million reads
MiSeq i100 Plus 100M Reagent Kit (300 cycles) Flowcell $1,400 100 million reads


Long Read

Nanopore (P2 Solo/promethION)

Item Unit Price Per Unit Notes
Nanopore Flowcell $1200 Upto 50 to 70GB data per flow cell


Pacbio

Item Unit Price Per Unit Notes
Pacbio SMRTCell 25 Million Run $2250 Around 6 million reads, outsourcing to sister facilities to enable access to the REVIO


RNA Library Prep

Standard (Manual)

Item Unit Price Per Unit Notes
Watchmaker QiaSeq FastSelect Ribodepletion, samples 1-12 Sample $200
Watchmaker QiaSeq FastSelect Ribodepletion, samples 13-24 Sample $185
Watchmaker QiaSeq FastSelect Ribodepletion, samples 25-48 Sample $135
Watchmaker QiaSeq FastSelect Ribodepletion, samples >48 Sample $100
Watchmaker mRNA, samples 1-12 Sample $150
Watchmaker mRNA, samples 13-24 Sample $135
Watchmaker mRNA, samples 25-48 Sample $85
Watchmaker mRNA, samples >48 Sample $50
Agilent SureSelect All Exon Sample $650
NEBNext Small RNA Sample $200



Low Input (Manual)

Item Unit Price Per Unit Notes
Clontech SMARTer UltraLow RNA w/ NexteraXT Sample $300



Highthroughput (96 well Plate Based)

Item Unit Price Per Unit Notes
HT mRNA library prep (Watchmaker) 48 Samples $2000 Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.

Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.

Libraries are pooled by FA quantification, expect variation in read balancing. 

96 samples $4000
Alithea Genomics DRUG-Seq (3') 24 Samples $1500 3' biased RNA-Seq - One Read per RNA molecule
48 samples $2000
72 samples $2500
96 samples $3000
Alithea Genomics DRUG-Seq (Full Length) 24 Samples $1800 RNA-Seq - Reads across the whole RNA molecule
48 samples $2300
72 samples $2800
96 samples $3300
Alithea Genomics FLASH-Seq 96 Samples $4500 Single cell plate-based RNA-Seq


Single Cell (10X and others)

Item Unit Price Per Unit Notes
10X Single Cell 3’ GEX (Includes OCM if requested) sample $2560
10X Single Cell 5’ GEX sample $2560
10X VDJ Add-on sample $230 Can be added onto the 10X 5' GEX Only
10X CRISPR Add-on sample $280 Can be added onto the 10X 5' GEX Only
10X Antibody/CellPlex/MultiSeq Add-on sample $140
10X Multiome Assay (ATAC + 3') sample $4200
10X Flex (Fixed Cell) sample $2800 Human and Mouse Only
Alithea Single Cell 96 well plates (FLASH-Seq) 96 well plate $4500 1 cell per well


DNA Library Prep

Item Unit Price Per Unit Notes
Nextera gDNA (Genomic, ATAC-Seq) sample $200 "Tagmentation" prep from intact gDNA (50ng)
Illumina DNA Prep (Genomic) sample $200 Genomic DNA >100ng


RNA Isolation

Item Unit Price Per Unit Notes
RNA-miRNeasy Total RNA Isolation 1-12 samples sample $85
RNA-miRNeasy Total RNA Isolation 12-24 samples sample $55
RNA-miRNeasy Total RNA Isolation 25-48 samples sample $35
RNA-miRNeasy Total RNA Isolation >48 samples sample $25
RNA - MagMax mirVana RNA Isolation sample $30
RNA - Direct-zol™-96 MagBead RNA Isolation sample $30


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer/Fragment Analyzer 1 to 20 Samples $15 Sizing and purity analysis
Fragment Analyzer (half 96 well plate) 20 to 48 Samples $300 Sizing and purity analysis
Fragment Analyzer (Full 96 well plate) 49+ Samples $500 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Sizing/Cleanup

Item Unit Price Per Unit Notes
Bead Sizing (Pippin) Up to 12 samples Sample $25
Bead Sizing (Pippin) Over 12 samples Sample $15
Gel Sizing (Pippin) Up to 12 samples Sample $50
Gel Sizing (Pippin) Over 12 samples Sample $25


Quantitative PCR - QuantStudio 6

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7 -
Equipment Time (2 hour time block) Plate $50 Sign-up Required


Digital PCR

Item Unit Price Per Unit Notes
Equipment Time (2 hour time block) Plate $50 Sign-up Required
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