Difference between revisions of "Pricing"

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Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
 
Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
 
{{PricingHeader}}
 
{{PricingHeader}}
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{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,140|}}
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{{PricingRow|HiSeq - 50 bp Single Read|Lane|$1,200|}}
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{{PricingRow|HiSeq - 26x40 10X|Lane|$1,300|ONLY FOR 10X LIBRARIES}}
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{{PricingRow|HiSeq - 28x40 10X|Lane|$1,500|ONLY FOR 10X LIBRARIES}}
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{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,710|}}
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{{PricingRow|HiSeq - 60x60 Paired End|Lane|$1,750|}}
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{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,260|}}
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{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,250|}}
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{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,700|}}
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{{PricingRow|HiSeq - Additional 70 bases|Lane|$400|Add to above base prices for longer reads}}
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{{PricingRow|HiSeq - Additional 40 bases|Lane|$330|Add to above base prices for longer reads}}
 
  
 
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In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250, and complete more quickly. For example - a 100x100 run in Rapid mode completes in 2-3 days vs up to 7 days in Standard mode.
 
In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250, and complete more quickly. For example - a 100x100 run in Rapid mode completes in 2-3 days vs up to 7 days in Standard mode.
 
{{PricingHeader}}
 
{{PricingHeader}}
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{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$1,680|}}
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{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$2,300|}}
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{{PricingRow|HiSeqRapid - 40 bp Single Read|1 lane|$1,140|}}
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{{PricingRow|HiSeqRapid - 28x40 10X|full flowcell (2 lanes)|$2,800|ONLY FOR 10X LIBRARIES}}
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{{PricingRow|HiSeqRapid - 26x40 10X|full flowcell (2 lanes)|$2,400|ONLY FOR 10X LIBRARIES}}
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{{PricingRow|HiSeqRapid - 60x60 Paired End|full flowcell (2 lanes)|$3,400|}}
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{{PricingRow|HiSeqRapid - 50x50 Paired End|full flowcell (2 lanes)|$2,500|}}
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{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$4,350|}}
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{{PricingRow|HiSeqRapid - 50x50 Paired End|1 lane|$1,500|}}
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{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$7,200|}}
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{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$3,250|}}
 
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{{PricingRow|HiSeqRapid - 100x100 Paired End|1 lane|$1,850|}}
 
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{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$5,550|}}
 
 
{{PricingRow| | | | }}
 
{{PricingRow| | | | }}
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{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$450| ONLY required if running different samples in each lane}}
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{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$460| ONLY required if running different samples in each lane}}
 
{{PricingRow| | | | }}
 
{{PricingRow| | | | }}
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{{PricingRow|HiSeqRapid - Additional 50 bases|full flowcell (2 lanes)|$500|Add to above base prices for longer reads up to 250x250}}
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{{PricingRow|HiSeqRapid - Additional 70 bases|full flowcell (2 lanes)|$560|Add to above base prices for longer reads up to 250x250}}
 
{{PricingLastRow||||}}
 
{{PricingLastRow||||}}
  
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{{PricingRow|Clontech Stranded Total RNA Pico (RNA-Seq)|Sample|$250|-}}
 
{{PricingRow|Clontech Stranded Total RNA Pico (RNA-Seq)|Sample|$250|-}}
 
{{PricingRow|NEBNext Small RNA (microRNA)|Sample|$200|-}}
 
{{PricingRow|NEBNext Small RNA (microRNA)|Sample|$200|-}}
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{{PricingRow|[http://jura.wi.mit.edu/genomecorewiki/index.php/SingleCell 10X Single Cell 3’ Solution Library prep (RNA-Seq)]|Sample|$1800|Sorting and library prep for up to 10,000 cells}}
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{{PricingRow|[http://jura.wi.mit.edu/genomecorewiki/index.php/SingleCell 10X Single Cell 3’ Solution Library prep (RNA-Seq)]|Sample|$2000|Sorting and library prep for up to 10,000 cells}}
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{{PricingLastRow|[http://jura.wi.mit.edu/genomecorewiki/index.php/SingleCell SMART-seq v2 Library prep (RNA-Seq)]|96-well Plate|$2400|}}
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{{PricingLastRow|[http://jura.wi.mit.edu/genomecorewiki/index.php/SingleCell SMART-seq v2 Library prep (RNA-Seq)]|96-well Plate|$2500|}}
  
 
<br>
 
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= Microarray Services =
 
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Prices for sample processing do not include cost of arrays
 
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== Affymetrix Array Processing==
 
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{{PricingHeader}}
 
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{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
 
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{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}
 
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<br>
 
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== Agilent Array Processing==
 
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{{PricingHeader}}
 
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{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
 
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{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
 
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{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}
 
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<br>
 
  
 
= nCounter Analysis System (NanoString) =
 
= nCounter Analysis System (NanoString) =

Revision as of 14:11, 12 July 2019

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Illumina Sequencing

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

HiSeq (Std a.k.a. "High Output" mode) -- At least 150-200 million reads per lane

Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

Item Unit Price Per Unit Notes
HiSeq - 50 bp Single Read Lane $1,200
HiSeq - 28x40 10X Lane $1,500 ONLY FOR 10X LIBRARIES
HiSeq - 60x60 Paired End Lane $1,750
HiSeq - 100x100 Paired End Lane $2,250
HiSeq - Additional 70 bases Lane $400 Add to above base prices for longer reads


HiSeq (Rapid) -- At least 100 to 150 million reads per lane (Two lanes per Flow Cell)

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250, and complete more quickly. For example - a 100x100 run in Rapid mode completes in 2-3 days vs up to 7 days in Standard mode.

Item Unit Price Per Unit Notes
HiSeqRapid - 50 bp Single Read full flowcell (2 lanes) $2,300
HiSeqRapid - 28x40 10X full flowcell (2 lanes) $2,800 ONLY FOR 10X LIBRARIES
HiSeqRapid - 60x60 Paired End full flowcell (2 lanes) $3,400
HiSeqRapid - 100x100 Paired End full flowcell (2 lanes) $4,350
HiSeqRapid - 250x250 Paired End full flowcell (2 lanes) $7,200
HiSeqRapid - Add DUAL mode full flowcell (2 lanes) $460 ONLY required if running different samples in each lane
HiSeqRapid - Additional 70 bases full flowcell (2 lanes) $560 Add to above base prices for longer reads up to 250x250


Library Prep

Item Unit Price Per Unit Notes
Swift ChIP DNA (ChIP-Seq) Sample $200 Optimized for low input ChIP
TruSeq Nano gDNA (Genomic) Sample $200 Genomic DNA >100ng
Nextera gDNA (Genomic, ATAC-Seq) Sample $200 "Tagmentation" prep from intact gDNA (50ng)
KAPA Hyper Prep - Stranded (RNA-Seq) Sample $200
Clontech UltraLow RNA (RNA-Seq) Sample $300 For low input RNA down to single cell
Clontech Stranded Total RNA Pico (RNA-Seq) Sample $250 -
NEBNext Small RNA (microRNA) Sample $200 -
10X Single Cell 3’ Solution Library prep (RNA-Seq) Sample $2000 Sorting and library prep for up to 10,000 cells
SMART-seq v2 Library prep (RNA-Seq) 96-well Plate $2500


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer Sample $15 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run



nCounter Analysis System (NanoString)

Item Unit Price Per Unit Notes
Equipment Use Cartridge $250 12 samples per cartridge
Reagents Cartridge $280.50* *Does NOT include CodeSet
Labor-Hyb Cartridge $150.00 -
Labor-miRNA Sample $25.00 -


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - QuantStudio 6

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7.00 -
Equipment Time (2 hour time block) Plate $30.00 Sign-up Required


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