NanoString - Revision history

Volkert at 21:43, 21 September 2010

2010-09-21T21:43:21Z

2010-09-21T21:42:37Z

2010-09-21T21:36:20Z

2010-09-21T19:46:49Z

2010-09-21T19:17:07Z

2010-09-21T19:16:59Z

2010-09-21T19:16:47Z

2010-09-21T19:16:02Z

2010-09-21T16:43:27Z

2010-09-21T16:17:01Z

← Older revision		Revision as of 21:43, 21 September 2010
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−	The nCounter™ Analysis System measures transcript abundance by tagging and counting individual mRNA molecules using a novel fluorescent barcoding technology. The system is able to detect 1 copy of mRNA per cell, can measure up to 800 genes per reaction, and requires only 10-100 ng of total RNA for analysis. ~~the~~ nCounter can also be used for miRNA profiling and CNV analysis.	+	The nCounter™ Analysis System measures transcript abundance by tagging and counting individual mRNA molecules using a novel fluorescent barcoding technology. The system is able to detect 1 copy of mRNA per cell, can measure up to 800 genes per reaction, and requires only 10-100 ng of total RNA for analysis. The nCounter can also be used for miRNA profiling and CNV analysis.

	Highlights:		Highlights:

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-The nCounter™ Analysis System measures abundance by tagging with barcodes and counting individual mRNA molecules using a novel digital technology. This system is able to detect 1 copy of mRNA per cell, can evaluate up to 800 genes per reaction, and requires only 10-100 ng of total RNA for analysis. Nanostrings can also be used for miRNA profiling and CNV analysis.
+The nCounter™ Analysis System measures transcript abundance by tagging and counting individual mRNA molecules using a novel fluorescent barcoding technology. The system is able to detect 1 copy of mRNA per cell, can measure up to 800 genes per reaction, and requires only 10-100 ng of total RNA for analysis. the nCounter can also be used for miRNA profiling and CNV analysis.
 Highlights:
-* More time-efficient and cost-effective than qPCR, if you have more than 10 genes and large number of samples
+* Fills the throughput niche between qPCR and microarrays for "mid-multiplex" assays
-* Custom probes designed by Nanostring for your selected regions
+* Ideal for assays of dozens to hundreds of genes in 50+ samples
-* No amplification
+* More time-efficient and cost-effective than qPCR
-* Digital detection
+* Direct measurement of transcript abundance (No amplification, digital readout)

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 == Sample Submission/Preparation ==
-New users should contact WIGTC for training and consultation. Custom codesets are designed by Nanostring specifically for your assay. Codeset pricing varies with number of targets and samples.
+New users should contact WIGTC for training and consultation. Custom CodeSets* are designed by Nanostring specifically for your assay. CodeSet pricing varies with number of targets and samples. Delivery times for CodeSet design and synthesis can be several weeks.
-Typically 100 ng of high quality total RNA is required for analysis using NanoStrings. Also, a codeset would have to be ordered before the experiment (CodeSet is the collection of all reporter probes and capture probes for all genes of interest in an assay).
+Typically 100 ng of total RNA is recommended though the assay is very tolerant to smaller inputs or partially degraded samples such as from FFPE.
 == Data ==

← Older revision		Revision as of 19:46, 21 September 2010
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	New users should contact WIGTC for training and consultation. Custom codesets are designed by Nanostring specifically for your assay. Codeset pricing varies with number of targets and samples.		New users should contact WIGTC for training and consultation. Custom codesets are designed by Nanostring specifically for your assay. Codeset pricing varies with number of targets and samples.
		+
		+	Typically 100 ng of high quality total RNA is required for analysis using NanoStrings. Also, a codeset would have to be ordered before the experiment (CodeSet is the collection of all reporter probes and capture probes for all genes of interest in an assay).

	== Data ==		== Data ==

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 === Is there documentation available about analyzing my data? ===
 Yes, there is a data analysis guidelines document that is available in the Product Literature from Nanostring. You can download it here:
 == Pricing ==
 Please refer to the [[Pricing]] Section

← Older revision		Revision as of 19:16, 21 September 2010
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	=== Is there documentation available about analyzing my data? ===		=== Is there documentation available about analyzing my data? ===
	Yes, there is a data analysis guidelines document that is available in the Product Literature from Nanostring. You can download it here:		Yes, there is a data analysis guidelines document that is available in the Product Literature from Nanostring. You can download it here:
−
−
−	~~== Turnaround Time ==~~

← Older revision		Revision as of 19:16, 21 September 2010
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−	== Sample Submission ~~Guidelines~~ ==	+	== Sample Submission/Preparation ==

	New users should contact WIGTC for training and consultation. Custom codesets are designed by Nanostring specifically for your assay. Codeset pricing varies with number of targets and samples.		New users should contact WIGTC for training and consultation. Custom codesets are designed by Nanostring specifically for your assay. Codeset pricing varies with number of targets and samples.