Sgupta at 16:49, 12 June 2009

2009-06-12T16:49:48Z

Sgupta at 16:44, 12 June 2009

2009-06-12T16:44:17Z

New page

'''SAMPLES NEEDING A CONTROL LANE'''

Unbalanced samples typically come from methylation sequencing, small RNA sequencing, ChIP sequencing, or sequencing of genomes with a high or low GC content. Experiments and samples that need a control lane are listed in Table 2. In general, most DNA and RNA sequencing experiments of mammalian genomes do not need a separate control lane for corrections, though a control lane could be used for troubleshooting purposes and as a quality reference point. If you want to run a number of samples and you are not sure whether to use a control lane, perform a run with a control lane and compare analysis with and without the control lane. Based on that, you can decide whether to use a control lane for subsequent similar experiments.

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 '''SAMPLES NEEDING A CONTROL LANE'''
-Unbalanced samples typically come from methylation sequencing, small RNA sequencing, ChIP sequencing, or sequencing of genomes with a high or low GC content. Experiments and samples that need a control lane are listed in Table 2. In general, most DNA and RNA sequencing experiments of mammalian genomes do not need a separate control lane for corrections, though a control lane could be used for troubleshooting purposes and as a quality reference point. If you want to run a number of samples and you are not sure whether to use a control lane, perform a run with a control lane and compare analysis with and without the control lane. Based on that, you can decide whether to use a control lane for subsequent similar experiments.
+Unbalanced samples typically come from methylation sequencing, small RNA sequencing, ChIP sequencing, or sequencing of genomes with a high or low GC content. Experiments and samples that need a control lane are listed in Table below. In general, most DNA and RNA sequencing experiments of mammalian genomes do not need a separate control lane for corrections, though a control lane could be used for troubleshooting purposes and as a quality reference point. If you want to run a number of samples and you are not sure whether to use a control lane, perform a run with a control lane and compare analysis with and without the control lane. Based on that, you can decide whether to use a control lane for subsequent similar experiments.
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ControlLane - Revision history

Sgupta at 16:49, 12 June 2009

Sgupta at 16:44, 12 June 2009