Genome Technology Core (GTC) wiki - Sequencing and Microarray - User contributions [en]

Pricing

2022-05-05T18:20:40Z

Volkert:

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for Illumina sequencing. NovaSeq, MiSeq, HiSeq "Standard" and HiSeq "Rapid".

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.

=== NovaSeq ===

{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
|-
| Item || Read Length || Unit || Price Per Unit || Notes
|-
|rowspan="4"|NovaSeqSP
| 100 Single End or 50x50 Paired-End
|rowspan="4"|Flow Cell (2 Lanes)
|$3,365
|rowspan="4"|~650–800 million reads per flowcell ~325-400 million reads per lane (x2 for Paired-End)
|-
| 200 Single End or 100x100 Paired-End
|$4,150
|-
| 300 Single End or 150x150 Paired-End
|$5,200
|-
| 500 Single End or 250x250 Paired-End
|$6,400
|-
|rowspan="3"|NovaSeqS1
| 100 Single End or 50x50 Paired-End
|rowspan="3"|Flow Cell (2 Lanes)
|$5,300
|rowspan="3"|~1.3–1.6 billion reads per flowcell ~650–800 million reads per lane (x2 for Paired-End)
|-
| 200 Single End or 100x100 Paired-End
|$6,900
|-
| 300 Single End or 150x150 Paired-End
|$7,750
|-
|rowspan="3"|NovaSeqS2
| 100 Single End or 50x50 Paired-End
|rowspan="3"|Flow Cell (2 Lanes)
|$9,500
|rowspan="3"|~3.3–4.1 billion reads per flowcell ~1.65-2.05 billion reads per lane (x2 for Paired-End)
|-
| 200 Single End or 100x100 Paired-End
|$11,500
|-
| 300 Single End or 150x150 Paired-End
|$12,600
|-
|rowspan="2"|NovaSeqS4
| 35 Single End
|rowspan="2"|Flow Cell (4 Lanes)
|$14,000
|rowspan="2"|~8-10 billion reads per flowcell ~2-2.5 billion reads per lane (x2 for Paired-End)
|-
| 300 Single End or 150x150 Paired-End
|$20,000
|-
|Add XP protocol - SP,S1,S2
|Any Read Length
|Flow Cell
|$400
|Allows different library pools in each lane
|-
|Add XP protocol - S4
|Any Read Length
|Flow Cell
|$1,200
|Allows different library pools in each lane
|-
|}

 

=== MiSeq -- 20 million reads ===
{{PricingHeader}}
{{PricingRow|MiSeq v3 150 Single End or 75x75 Paired End|Flowcell|$1,485|}}
{{PricingLastRow||||}}
 

=== HiSeq (Std a.k.a. "High Output" mode) -- At least 150-200 million reads per lane ===
Standard mode require 8 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
{{PricingHeader}}
{{PricingRow|HiSeq - 50 bp Single Read|Lane|$1,200|}}
{{PricingRow|HiSeq - Additional 70 bases|Lane|$400|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) -- At least 100 to 150 million reads per lane (Two lanes per Flow Cell) ===
In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250, and complete more quickly. For example - a 100x100 run in Rapid mode completes in 2-3 days vs up to 7 days in Standard mode.
{{PricingHeader}}
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$2,300|}}
{{PricingRow|HiSeqRapid - 28x40 10X|full flowcell (2 lanes)|$2,800|ONLY FOR 10X LIBRARIES}}
{{PricingRow|HiSeqRapid - 60x60 Paired End|full flowcell (2 lanes)|$3,400|}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$4,350|}}
{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$7,200|}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$460| ONLY required if running different samples in each lane}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Additional 70 bases|full flowcell (2 lanes)|$560|Add to above base prices for longer reads up to 250x250}}
{{PricingLastRow||||}}

 

= Library Prep =
{{PricingHeader}}
{{PricingRow|Swift ChIP DNA (ChIP-Seq)|Sample|$200|Optimized for low input ChIP}}
{{PricingRow|Illumina DNA (Genomic)|Sample|$200|Genomic DNA >100ng}}
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|Sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
{{PricingRow|KAPA Hyper Prep - Stranded (RNA-Seq)|Sample|$200|}}
{{PricingRow|KAPA Ribo Erase (RNA-Seq)|Sample|$250|}}
{{PricingRow|Clontech UltraLow RNA (RNA-Seq)|Sample|$300|For low input RNA down to single cell}}
{{PricingRow|Clontech Stranded Total RNA (RNA-Seq)|Sample|$250|-}}
{{PricingRow|NEBNext Small RNA (microRNA)|Sample|$200|-}}
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 3’ Solution Library prep (RNA-Seq)]|Sample|$2,205|Sorting and library prep for up to 10,000 cells}}
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 5’ Solution Library prep (RNA-Seq)]|Sample|$2,363|Sorting and library prep for up to 10,000 cells}}
{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/SingleCell SMART-seq v2 Library prep (RNA-Seq)]|96-well Plate|$2,500|}}

{| style="background:white; color:black;" align="right"
| [[LibraryPrep|Click here for more info about Library Prep options]]
|}
 

= Quality Control =
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Reagents|Cartridge|----|Reagents and Codesets Purchase From Nanostring}}
{{PricingRow|Equipment Use|Cartridge|$250|12 samples per cartridge}}
{{PricingRow|Labor-Hyb|Cartridge|$150|-}}
{{PricingLastRow|Labor-miRNA|Sample|$25|Addon for miRNA protocol only}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - QuantStudio 6 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30|Sign-up Required}}

Pricing

2021-06-02T13:21:47Z

Volkert: /* Library Prep */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for Illumina sequencing. NovaSeq, HiSeq "Standard" and HiSeq "Rapid".

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.

=== NovaSeq ===

{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
|-
| Item || Read Length || Unit || Price Per Unit || Notes
|-
|rowspan="4"|NovaSeqSP
| 100 Single End or 50x50 Paired-End
|rowspan="4"|Flow Cell (2 Lanes)
|$3,365
|rowspan="4"|~650–800 million reads per flowcell ~325-400 reads per lane (x2 for Paired-End)
|-
| 200 Single End or 100x100 Paired-End
|$4,150
|-
| 300 Single End or 150x150 Paired-End
|$5,200
|-
| 500 Single End or 250x250 Paired-End
|$6,400
|-
|rowspan="3"|NovaSeqS1
| 100 Single End or 50x50 Paired-End
|rowspan="3"|Flow Cell (2 Lanes)
|$5,300
|rowspan="3"|~1.3–1.6 billion reads per flowcell ~650–800 million reads per lane (x2 for Paired-End)
|-
| 200 Single End or 100x100 Paired-End
|$6,900
|-
| 300 Single End or 150x150 Paired-End
|$7,750
|-
|rowspan="3"|NovaSeqS2
| 100 Single End or 50x50 Paired-End
|rowspan="3"|Flow Cell (2 Lanes)
|$9,500
|rowspan="3"|~3.3–4.1 billion reads per flowcell ~1.65-2.05 billion reads per lane (x2 for Paired-End)
|-
| 200 Single End or 100x100 Paired-End
|$11,500
|-
| 300 Single End or 150x150 Paired-End
|$12,600
|-
|rowspan="2"|NovaSeqS4
| 35 Single End
|rowspan="2"|Flow Cell (4 Lanes)
|$14,000
|rowspan="2"|~8-10 billion reads per flowcell ~2-2.5 billion reads per lane (x2 for Paired-End)
|-
| 300 Single End or 150x150 Paired-End
|$20,000
|-
|Add XP protocol - SP,S1,S2
|Any Read Length
|Flow Cell
|$400
|Allows different library pools in each lane
|-
|Add XP protocol - S4
|Any Read Length
|Flow Cell
|$1,200
|Allows different library pools in each lane
|-
|}

 

=== HiSeq (Std a.k.a. "High Output" mode) -- At least 150-200 million reads per lane ===
Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
{{PricingHeader}}
{{PricingRow|HiSeq - 50 bp Single Read|Lane|$1,200|}}
{{PricingRow|HiSeq - 28x40 10X|Lane|$1,500|ONLY FOR 10X LIBRARIES}}
{{PricingRow|HiSeq - 60x60 Paired End|Lane|$1,750|}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,250|}}
{{PricingRow|HiSeq - Additional 70 bases|Lane|$400|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) -- At least 100 to 150 million reads per lane (Two lanes per Flow Cell) ===
In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250, and complete more quickly. For example - a 100x100 run in Rapid mode completes in 2-3 days vs up to 7 days in Standard mode.
{{PricingHeader}}
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$2,300|}}
{{PricingRow|HiSeqRapid - 28x40 10X|full flowcell (2 lanes)|$2,800|ONLY FOR 10X LIBRARIES}}
{{PricingRow|HiSeqRapid - 60x60 Paired End|full flowcell (2 lanes)|$3,400|}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$4,350|}}
{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$7,200|}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$460| ONLY required if running different samples in each lane}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Additional 70 bases|full flowcell (2 lanes)|$560|Add to above base prices for longer reads up to 250x250}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|Swift ChIP DNA (ChIP-Seq)|Sample|$200|Optimized for low input ChIP}}
{{PricingRow|TruSeq Nano gDNA (Genomic)|Sample|$200|Genomic DNA >100ng}}
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|Sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
{{PricingRow|KAPA Hyper Prep - Stranded (RNA-Seq)|Sample|$200|}}
{{PricingRow|KAPA Ribo Erase (RNA-Seq)|Sample|$250|}}
{{PricingRow|Clontech UltraLow RNA (RNA-Seq)|Sample|$300|For low input RNA down to single cell}}
{{PricingRow|Clontech Stranded Total RNA (RNA-Seq)|Sample|$250|-}}
{{PricingRow|NEBNext Small RNA (microRNA)|Sample|$200|-}}
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 3’ Solution Library prep (RNA-Seq)]|Sample|$2,100|Sorting and library prep for up to 10,000 cells}}
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 5’ Solution Library prep (RNA-Seq)]|Sample|$2,250|Sorting and library prep for up to 10,000 cells}}
{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/SingleCell SMART-seq v2 Library prep (RNA-Seq)]|96-well Plate|$2,500|}}

{| style="background:white; color:black;" align="right"
| [[LibraryPrep|Click here for more info about Library Prep options]]
|}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Reagents|Cartridge|----|Reagents and Codesets Purchase From Nanostring}}
{{PricingRow|Equipment Use|Cartridge|$250|12 samples per cartridge}}
{{PricingRow|Labor-Hyb|Cartridge|$150|-}}
{{PricingLastRow|Labor-miRNA|Sample|$25|Addon for miRNA protocol only}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - QuantStudio 6 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30|Sign-up Required}}

Main Page

2021-04-08T18:25:34Z

Volkert:

{| style="font-style:italic; font-size:120%; width:100%; border:2px solid white; height:100px" align="center"
|-
| [[image:Lab-Panorama-shot-web.jpg|center]]
|-
|}
{| style="border:1px solid red; color: white; text-align: center; width:800px" align="center"
|-
| <h6 align="center"> The Genome Technology Core is open and accepting samples for most services. Samples may be submitted as usual through the [http://gtc.wi.mit.edu/apps/ online portal]. Internal customers may leave samples in the [[Special:Redirect/file/dropoff.png|drop box in the -80C freezer in the hallway outside room 325]]. External customers should mail samples or contact GTC staff to schedule a sample exchange at the building entrance. 

The Real-time PCR machines and Covaris system have been moved to room 360. Please reserve 2 hour blocks for these instruments on our resource scheduling calendar. ([[Calendar|Resource Scheduling Systems for RT PCR and Covaris]]). The Nanodrop and Qubit systems are also in room 360 and are available for walk-up use as long as nobody else is in the room. Only one person in room 360 at a time please.
</h6>
|-
|}
{| style="background:white; color:black; width:800px" align="center"
|
{|
|
{| style="border:1px solid purple; width:800px; height:100px; text-align: center" align="center"
| valign="top" | Services
{| style="text-align: left; width:100%"
|
[[Pricing|'''CLICK HERE FOR PRICE LIST''']]

Service Details:
* [[Sequencing|Sequencing]]
* [[LibraryPrep|Library Prep]]
* [[SingleCell|Single Cell Sequencing]]
* [[NanoString|NanoString]]
* [[RT_PCR|Real-Time PCR]]
* Bioanalyzer
|}
|}
{| style="background:white; color:black; width:800px" align="center"
|
{| style="border:1px solid purple; width:400px; height:140px; text-align: center"
| valign="top" | Hot Links
{| style="text-align: left; width:100%"
|
* [https://cores.wi.mit.edu/ External Lab Customer Registration]
* [[Forms|Sample Submission Forms (All Services)]]
* [[SubmissionGuide|Sequencing Sample Submission Guide]]
* [[Calendar|Resource Scheduling Systems for RT PCR and Covaris]]
* [[faq|Frequently Asked Questions]]
|}
|}
||
{| style="border:1px solid purple; width:400px; height:140px; text-align: center"
| valign="top" | Data Related Resources
{| style="text-align: left; width:100%"
|
* [[SequencingQC|Sequencing QC]]
* [[SequencingFormats|Sequencing Formats]]
* Analysis - [[Scripts|Scripts]], [[Data_Analysis_Resources|Other Resources]]
* [[General_Links_Resources|General Links]]
* [[NCBISubmission|NCBI Submission Guide]]
|}
|}
|
|}
{| style="background:white; color:black; width:800px" align="center"
|
{| style="border:1px solid purple; width:400px; height:120px; text-align: center"
| valign="top" | Contact Us
{| style="text-align: center; width:100%"
|
455 Main Street, 
Cambridge, MA - 02142 
Tel: 617-258-8803 
[[wibr-genome at wi dot mit dot edu]]
|}
|}
||
{| style="border:1px solid purple; width:400px; height:120px; text-align: center"
| valign="top" | Other General Information
{| style="text-align: left; width:100%"
|
* [[Personnel]] - [[Tom_Volkert|Tom Volkert]], [[Jennifer_Love|Jennifer Love]], [[Sumeet_Gupta|Sumeet Gupta]], Stephen Mraz III, Amanda Chilaka
|}
|}
|
|}
|}
|}

Main Page

2021-04-08T18:23:44Z

Volkert:

{| style="font-style:italic; font-size:120%; width:100%; border:2px solid white; height:100px" align="center"
|-
| [[image:Lab-Panorama-shot-web.jpg|center]]
|-
|}
{| style="border:1px solid red; color: white; text-align: center; width:800px" align="center"
|-
| <h6 align="center"> The Genome Technology Core is open and accepting samples for most services. Samples may be submitted as usual through the [http://gtc.wi.mit.edu/apps/ online portal]. Internal customers may leave samples in the [[Special:Redirect/file/dropoff.png|drop box in the -80C freezer in the hallway outside room 325]]. External customers should mail samples or contact GTC staff to schedule a sample exchange at the building entrance. 

The Real-time PCR machines and Covaris system have been moved to room 360. Please reserve 2 hour blocks for these instruments on our resource scheduling calendar. ([[Calendar|Resource Scheduling Systems for RT PCR and Covaris]]). The Nanodrop and Qubit systems are also in room 360 and are available for walk-up use as long as nobody else is in the room. Only one person in room 360 at a time please.
</h6>
|-
|}
{| style="background:white; color:black; width:800px" align="center"
|
{|
|
{| style="border:1px solid purple; width:800px; height:100px; text-align: center" align="center"
| valign="top" | Services
{| style="text-align: left; width:100%"
|
[[Pricing|'''CLICK HERE FOR PRICELIST''']]

Service Details:
* [[Sequencing|Sequencing]]
* [[LibraryPrep|Library Prep]]
* [[SingleCell|Single Cell Sequencing]]
* [[NanoString|NanoString]]
* [[RT_PCR|Real-Time PCR]]
* Bioanalyzer
|}
|}
{| style="background:white; color:black; width:800px" align="center"
|
{| style="border:1px solid purple; width:400px; height:140px; text-align: center"
| valign="top" | Hot Links
{| style="text-align: left; width:100%"
|
* [https://cores.wi.mit.edu/ External Lab Customer Registration]
* [[Forms|Sample Submission Forms (All Services)]]
* [[SubmissionGuide|Sequencing Sample Submission Guide]]
* [[Calendar|Resource Scheduling Systems for RT PCR and Covaris]]
* [[faq|Frequently Asked Questions]]
|}
|}
||
{| style="border:1px solid purple; width:400px; height:140px; text-align: center"
| valign="top" | Data Related Resources
{| style="text-align: left; width:100%"
|
* [[SequencingQC|Sequencing QC]]
* [[SequencingFormats|Sequencing Formats]]
* Analysis - [[Scripts|Scripts]], [[Data_Analysis_Resources|Other Resources]]
* [[General_Links_Resources|General Links]]
* [[NCBISubmission|NCBI Submission Guide]]
|}
|}
|
|}
{| style="background:white; color:black; width:800px" align="center"
|
{| style="border:1px solid purple; width:400px; height:120px; text-align: center"
| valign="top" | Contact Us
{| style="text-align: center; width:100%"
|
455 Main Street, 
Cambridge, MA - 02142 
Tel: 617-258-8803 
[[wibr-genome at wi dot mit dot edu]]
|}
|}
||
{| style="border:1px solid purple; width:400px; height:120px; text-align: center"
| valign="top" | Other General Information
{| style="text-align: left; width:100%"
|
* [[Personnel]] - [[Tom_Volkert|Tom Volkert]], [[Jennifer_Love|Jennifer Love]], [[Sumeet_Gupta|Sumeet Gupta]], Stephen Mraz III, Amanda Chilaka
|}
|}
|
|}
|}
|}

LibraryPrep

2020-08-31T20:52:26Z

Volkert: /* Swift 2S */

= Library prep =
== RNA ==
=== KAPA Hyperprep mRNA ===
The most common RNA kit option. Best for standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.

=== KAPA Hyperprep with Ribo-depletion ===
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)

=== Clonetech SMART-Seq v4 Ultra-low ===
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.

=== Clonetech SMARTer PICO ===
For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA.

=== NEBnext small RNA ===
For microRNA and other small RNA. 100-1000ng total RNA.

== DNA ==
=== Swift 2S ===
For ChIP and other low input DNA. For ChIP samples the input is enriched and purified dsDNA. There is no lower limit for input but odds of failure goes up with lower input. >10ng is generally fine.

=== Nextera gDNA ===
“Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.

== Frequently Asked Questions ==
Please refer to [[Faq#Bulk_RNA-Seq|frequently asked questions section for FAQ regarding]] library prep options.

Main Page

2020-08-31T20:49:15Z

Volkert:

{| style="font-style:italic; font-size:120%; width:100%; border:2px solid white; height:100px" align="center"
|-
| [[image:Lab-Panorama-shot-web.jpg|center]]
|-
|}
{| style="border:1px solid red; color: white; text-align: center; width:800px" align="center"
|-
| <h6 align="center"> The Genome Technology Core is open and accepting samples for most services. Samples may be submitted as usual through the [http://gtc.wi.mit.edu/apps/ online portal]. Internal customers may leave samples in the [[Special:Redirect/file/dropoff.png|drop box in the -80C freezer in the hallway outside room 325]]. External customers should mail samples or contact GTC staff to schedule a sample exchange at the building entrance. 

The Real-time PCR machines and Covaris system have been moved to room 360. Please reserve 2 hour blocks for these instruments on our resource scheduling calendar. ([[Calendar|Resource Scheduling Systems for RT PCR and Covaris]]). The Nanodrop and Qubit systems are also in room 360 and are available for walk-up use as long as nobody else is in the room. Only one person in room 360 at a time please.
</h6>
|-
|}
{| style="background:white; color:black; width:800px" align="center"
|
{|
|
{| style="border:1px solid purple; width:800px; height:100px; text-align: center" align="center"
| valign="top" | Services
{| style="text-align: left; width:100%"
|
[[Pricing|'''List of Services and Prices''']]

Service Details:
* [[Sequencing|Sequencing]]
* [[LibraryPrep|Library Prep]]
* [[SingleCell|Single Cell Sequencing]]
* [[NanoString|NanoString]]
* [[RT_PCR|Real-Time PCR]]
* Bioanalyzer
|}
|}
{| style="background:white; color:black; width:800px" align="center"
|
{| style="border:1px solid purple; width:400px; height:140px; text-align: center"
| valign="top" | Hot Links
{| style="text-align: left; width:100%"
|
* [https://cores.wi.mit.edu/ External Lab Customer Registration]
* [[Forms|Sample Submission Forms (All Services)]]
* [[SubmissionGuide|Sequencing Sample Submission Guide]]
* [[Calendar|Resource Scheduling Systems for RT PCR and Covaris]]
* [[faq|Frequently Asked Questions]]
|}
|}
||
{| style="border:1px solid purple; width:400px; height:140px; text-align: center"
| valign="top" | Data Related Resources
{| style="text-align: left; width:100%"
|
* [[SequencingQC|Sequencing QC]]
* [[SequencingFormats|Sequencing Formats]]
* Analysis - [[Scripts|Scripts]], [[Data_Analysis_Resources|Other Resources]]
* [[General_Links_Resources|General Links]]
* [[NCBISubmission|NCBI Submission Guide]]
|}
|}
|
|}
{| style="background:white; color:black; width:800px" align="center"
|
{| style="border:1px solid purple; width:400px; height:120px; text-align: center"
| valign="top" | Contact Us
{| style="text-align: center; width:100%"
|
455 Main Street, 
Cambridge, MA - 02142 
Tel: 617-258-8803 
[[wibr-genome at wi dot mit dot edu]]
|}
|}
||
{| style="border:1px solid purple; width:400px; height:120px; text-align: center"
| valign="top" | Other General Information
{| style="text-align: left; width:100%"
|
* [[Personnel]] - [[Tom_Volkert|Tom Volkert]], [[Jennifer_Love|Jennifer Love]], [[Sumeet_Gupta|Sumeet Gupta]], Stephen Mraz III, Amanda Chilaka
|}
|}
|
|}
|}
|}

Main Page

2020-05-27T15:29:27Z

Volkert: removed unnecessary text for cleaner look

{| style="font-style:italic; font-size:120%; width:100%; border:2px solid white; height:100px" align="center"
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| [[image:Lab-Panorama-shot-web.jpg|center]]
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{| style="border:1px solid red; color: white; text-align: center; width:800px" align="center"
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| <h3 align="center"> Due to COVID-19 prevention procedures we will be scaling back operations significantly for the next several weeks. Customers can continue to submit samples during this time but please be advised that there may be delays in processing. Additionally, external customers intending to deliver samples in person will need to arrange with core staff to meet you at the door for sample handoff as access to the building is restricted to Whitehead staff only. If you have specific questions you can email the core at wibr-genome@wi.mit.edu. </h3>
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{| style="background:white; color:black; width:800px" align="center"
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{|
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{| style="border:1px solid purple; width:800px; height:100px; text-align: center" align="center"
| valign="top" | Services
{| style="text-align: left; width:100%"
|
[[Pricing|List of Services and Prices]]

Service Details:
* [[Sequencing|Sequencing]]
* [[LibraryPrep|Library Prep]]
* [[SingleCell|Single Cell Sequencing]]
* [[NanoString|NanoString]]
* [[RT_PCR|Real-Time PCR]]
* Bioanalyzer
|}
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{| style="background:white; color:black; width:800px" align="center"
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{| style="border:1px solid purple; width:400px; height:140px; text-align: center"
| valign="top" | Hot Links
{| style="text-align: left; width:100%"
|
* [https://cores.wi.mit.edu/ External Lab Customer Registration]
* [[Forms|Sample Submission Forms (All Services)]]
* [[SubmissionGuide|Sequencing Sample Submission Guide]]
* [[Calendar|Resource Scheduling Systems for RT PCR]]
* [[faq|Frequently Asked Questions]]
|}
|}
||
{| style="border:1px solid purple; width:400px; height:140px; text-align: center"
| valign="top" | Data Related Resources
{| style="text-align: left; width:100%"
|
* [[SequencingQC|Sequencing QC]]
* [[SequencingFormats|Sequencing Formats]]
* Analysis - [[Scripts|Scripts]], [[Data_Analysis_Resources|Other Resources]]
* [[General_Links_Resources|General Links]]
* [[NCBISubmission|NCBI Submission Guide]]
|}
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{| style="background:white; color:black; width:800px" align="center"
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{| style="border:1px solid purple; width:400px; height:120px; text-align: center"
| valign="top" | Contact Us
{| style="text-align: center; width:100%"
|
455 Main Street, 
Cambridge, MA - 02142 
Tel: 617-258-8803 
[[wibr-genome at wi dot mit dot edu]]
|}
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{| style="border:1px solid purple; width:400px; height:120px; text-align: center"
| valign="top" | Other General Information
{| style="text-align: left; width:100%"
|
* [[Personnel]] - [[Tom_Volkert|Tom Volkert]], [[Jennifer_Love|Jennifer Love]], [[Sumeet_Gupta|Sumeet Gupta]], Stephen Mraz III, Amanda Chilaka
|}
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Pricing

2017-08-09T18:51:46Z

Volkert: /* Illumina Sequencing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 150-200 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to 10 days in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,140|avg 150-200 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,710|avg 150-200 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,260|avg 150-200 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,700|avg 150-200 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$330|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$1,680|avg 200-280 million reads}}
{{PricingRow|HiSeqRapid - 40 bp Single Read|1 lane|$1,140|avg 100-140 million reads}}
{{PricingRow|HiSeqRapid - 50x50 Paired End|full flowcell (2 lanes)|$2,500|avg 200-280 million paired end reads}}
{{PricingRow|HiSeqRapid - 50x50 Paired End|1 lane|$1,500|avg 100-140 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$3,250|avg 200-280 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|1 lane|$1,850|avg 100-140 million paired end reads}}
{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$5,550|avg 200-280 million paired end reads}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$450| ONLY required if running different samples in each lane}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Additional 50 bases|full flowcell (2 lanes)|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|Swift ChIP DNA (ChIP-Seq)|Sample|$200|Optimized for low input ChIP}}
{{PricingRow|TruSeq Nano gDNA (Genomic)|Sample|$200|Genomic DNA >100ng}}
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|Sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
{{PricingRow|TruSeq Stranded mRNA (RNA-Seq)|Sample|$200|Basic RNA Expression Analysis}}
{{PricingRow|TruSeq Stranded RiboZero (RNA-Seq)|Sample|$250|Replaces polyA selection with a ribozomal RNA depletion}}
{{PricingRow|Clontech UltraLow RNA (RNA-Seq)|Sample|$300|For low input RNA down to single cell}}
{{PricingRow|Clontech Stranded Total RNA Pico (RNA-Seq)|Sample|$250|-}}
{{PricingLastRow|NEBNext Small RNA (microRNA)|Sample|$200|-}}

 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$250|12 samples per cartridge}}
{{PricingRow|Reagents|Cartridge|$280.50*|*Does NOT include CodeSet}}
{{PricingRow|Labor-Hyb|Cartridge|$150.00|-}}
{{PricingLastRow|Labor-miRNA|Sample|$25.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - QuantStudio 6 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

File:Tom.jpg

2017-02-21T19:22:52Z

Volkert: uploaded a new version of "File:Tom.jpg": Reverted to version as of 19:11, 21 February 2017

Tom Snap

File:Tom.jpg

2017-02-21T19:14:24Z

Volkert: uploaded a new version of "File:Tom.jpg": Reverted to version as of 19:10, 21 February 2017

Tom Snap

File:Tom.jpg

2017-02-21T19:12:17Z

Volkert: uploaded a new version of "File:Tom.jpg": Reverted to version as of 19:10, 21 February 2017

Tom Snap

File:Tom.jpg

2017-02-21T19:11:08Z

Volkert: uploaded a new version of "File:Tom.jpg": Reverted to version as of 19:08, 21 February 2017

Tom Snap

File:Tom.jpg

2017-02-21T19:10:28Z

Volkert: uploaded a new version of "File:Tom.jpg"

Tom Snap

File:Tom.jpg

2017-02-21T19:08:33Z

Volkert: uploaded a new version of "File:Tom.jpg"

Tom Snap

File:Tom.jpg

2017-02-21T19:06:18Z

Volkert: uploaded a new version of "File:Tom.jpg"

Tom Snap

File:Tom.jpg

2017-02-21T19:04:34Z

Volkert: uploaded a new version of "File:Tom.jpg"

Tom Snap

File:Tom.jpg

2017-02-21T18:56:54Z

Volkert: uploaded a new version of "File:Tom.jpg"

Tom Snap

Pricing

2016-08-03T18:14:21Z

Volkert: /* HiSeq (Rapid) */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to 10 days in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,140|avg 120-150 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,710|avg 120-150 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,260|avg 120-150 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,700|avg 120-150 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$330|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$1,680|avg ~160-200 million reads}}
{{PricingRow|HiSeqRapid - 40 bp Single Read|1 lane|$1,140|avg ~80-100 million reads}}
{{PricingRow|HiSeqRapid - 50x50 Paired End|full flowcell (2 lanes)|$2,500|avg ~160-200 million paired end reads}}
{{PricingRow|HiSeqRapid - 50x50 Paired End|1 lane|$1,500|avg ~80-100 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$3,250|avg ~160-200 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|1 lane|$1,850|avg ~80-100 million paired end reads}}
{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$5,550|avg ~160-200 million paired end reads}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$450| ONLY required if running different samples in each lane}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Additional 50 bases|full flowcell (2 lanes)|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|TruSeq ChIP DNA|Sample|$200|}}
{{PricingRow|Swift ChIP DNA|Sample|$200|Optimized for low input ChIP}}
{{PricingRow|TruSeq Nano gDNA|Sample|$200|Genomic DNA >100ng}}
{{PricingRow|TruSeq Stranded mRNA|Sample|$200|Basic RNA Expression Analysis}}
{{PricingRow|TruSeq Stranded RiboZero|Sample|$250|Replaces polyA selection with a ribozomal RNA depletion}}
{{PricingRow|Clontech UltraLow RNA|Sample|$400|For low input RNA down to single cell}}
{{PricingRow|Nextera genomic DNA|Sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - QuantStudio 6 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2016-08-03T18:12:06Z

Volkert: /* HiSeq (Std) */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to 10 days in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,140|avg 120-150 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,710|avg 120-150 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,260|avg 120-150 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,700|avg 120-150 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$330|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$1,600|avg ~160-200 million reads}}
{{PricingRow|HiSeqRapid - 40 bp Single Read|1 lane|$1,085|avg ~80-100 million reads}}
{{PricingRow|HiSeqRapid - 50x50 Paired End|full flowcell (2 lanes)|$2,400|avg ~160-200 million paired end reads}}
{{PricingRow|HiSeqRapid - 50x50 Paired End|1 lane|$1,420|avg ~80-100 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$3,100|avg ~160-200 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|1 lane|$1,770|avg ~80-100 million paired end reads}}
{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$5,300|avg ~160-200 million paired end reads}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$440| ONLY required if running different samples in each lane}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Additional 50 bases|full flowcell (2 lanes)|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|TruSeq ChIP DNA|Sample|$200|}}
{{PricingRow|Swift ChIP DNA|Sample|$200|Optimized for low input ChIP}}
{{PricingRow|TruSeq Nano gDNA|Sample|$200|Genomic DNA >100ng}}
{{PricingRow|TruSeq Stranded mRNA|Sample|$200|Basic RNA Expression Analysis}}
{{PricingRow|TruSeq Stranded RiboZero|Sample|$250|Replaces polyA selection with a ribozomal RNA depletion}}
{{PricingRow|Clontech UltraLow RNA|Sample|$400|For low input RNA down to single cell}}
{{PricingRow|Nextera genomic DNA|Sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - QuantStudio 6 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

RT PCR

2016-03-16T18:37:52Z

Volkert:

The GTC features the QuantStudio 6 Real-Time PCR system from Applied Biosystems for quantitative PCR. This highly flexible system supports both 96 and 384 well plate formats for both Taqman and SYBR green applications. 

== Access and Availability ==

The GTC has two QuantStudio 6 systems from ABI. One is set up for 384 well plates, the other for 96 well plates.

Access is available in 2 hour time blocks 24 hours a day, 7 days a week. Advance sign-up is required. It is recommended that you sign up at least 48 hours in advance to ensure availability. 

New users should contact Jennifer Love [jlove at wi dot mit dot edu] to schedule an orientation session.
Please do not attempt to change the blocks unless you have been trained as improper handling can damage the blocks. Jennifer is available during normal business hours for training. 

Due to high demand, access to this equipment is limited to Whitehead Institute users only. 

==Sign-up policy==

We use a online sign-up system for the RT-PCR machines. '''Please Carefully Read The Following Before You Book A Slot''' 

* Reserved Slots/Blocks cannot be deleted AFTER the timeslot begins. 
* All Reserved Slots/Blocks will be charged (unless deleted from the system). 
* Overbooking will not be tolerated. If you are identified as someone reserving more slots than you need and then deleting them, you will be penalized for misusing the system and creating inconvenience for others. 
* Please ensure you complete your run within your allotted timeslots/blocks. 
* Logging reagent usage is mandatory for everyone using the machines. If no GTC reagents are used, please log the number of plates and uLmix as 0) 
* You will get multiple email reminders to log your reagent usage If reagent usage is not logged by the 10th of the following month we will bill for the maximum potential usage.
 
You can sign-up for the a time slot to use the machine online. [[Calendar|Click Here For Details]].
 

The following consumables and reagents are available from the GTC for a fee:
* Optical plates (96-well fast, 384-well)
* Optical Adhesive Films
* SYBR Green Master Mix

== Analysis ==

The analysis software SDS v2.3 is installed on our analysis computer located in room 352.

The installation disc is no longer available. You may download '''SDS software v2.4''' from [http://www.appliedbiosystems.com/absite/us/en/home/support/software/real-time-pcr/ab-7900ht.html here]

It is free and all you need is a serial number of the equipment. Please stop by for the number.

== Pricing ==
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|6.5 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

RT PCR

2016-03-16T18:35:18Z

Volkert: /* Access and Availability */

The GTC features the 7900HT Fast Real-Time PCR system from Applied Biosystems for quantitative PCR. This highly flexible system supports both 96 and 384 well plate formats as well as the TLDA (TaqMan Low Density Array) card format. 

[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=601255| Information about the 7900HT] 
[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600694| Information about the TLDA system can be found here] 

== Access and Availability ==

The GTC has two QuantStudio 6 systems from ABI. One is set up for 384 well plates, the other for 96 well plates.

Access is available in 2 hour time blocks 24 hours a day, 7 days a week. Advance sign-up is required. It is recommended that you sign up at least 48 hours in advance to ensure availability. 

New users should contact Jennifer Love [jlove at wi dot mit dot edu] to schedule an orientation session.
Please do not attempt to change the blocks unless you have been trained as improper handling can damage the blocks. Jennifer is available during normal business hours for training. 

Due to high demand, access to this equipment is limited to Whitehead Institute users only. 

==Sign-up policy==

We use a online sign-up system for the ABI 7900 machines. '''Please Carefully Read The Following Before You Book A Slot''' 

* Reserved Slots/Blocks cannot be deleted AFTER the timeslot begins. 
* All Reserved Slots/Blocks will be charged (unless deleted from the system). 
* Overbooking will not be tolerated. If you are identified as someone reserving more slots than you need and then deleting them, you will be penalized for misusing the system and creating inconvenience for others. 
* Please ensure you complete your run within your allotted timeslots/blocks. 
* Logging reagent usage is mandatory for everyone using the machines. If no GTC reagents are used, please log the number of plates and uLmix as 0) 
* You will get multiple email reminders to log your reagent usage If reagent usage is not logged by the 10th of the following month we will bill for the maximum potential usage.
 
You can sign-up for the a time slot to use the machine online. [[Calendar|Click Here For Details]].
 

The following consumables and reagents are available from the GTC for a fee:
* Optical plates (96-well fast, 384-well)
* Optical Adhesive Films
* SYBR Green Master Mix

== Analysis ==

The analysis software SDS v2.3 is installed on our analysis computer located in room 352.

The installation disc is no longer available. You may download '''SDS software v2.4''' from [http://www.appliedbiosystems.com/absite/us/en/home/support/software/real-time-pcr/ab-7900ht.html here]

It is free and all you need is a serial number of the equipment. Please stop by for the number.

== Pricing ==
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|6.5 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-07-20T16:16:00Z

Volkert: /* Library Prep */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to 10 days in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-150 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-150 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-150 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-150 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$1,600|avg ~160-200 million reads}}
{{PricingRow|HiSeqRapid - 40 bp Single Read|1 lane|$1,085|avg ~80-100 million reads}}
{{PricingRow|HiSeqRapid - 40x40 Paired End|full flowcell (2 lanes)|$2,400|avg ~160-200 million paired end reads}}
{{PricingRow|HiSeqRapid - 40x40 Paired End|1 lane|$1,420|avg ~80-100 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$3,100|avg ~160-200 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|1 lane|$1,770|avg ~80-100 million paired end reads}}
{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$5,300|avg ~160-200 million paired end reads}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$440| ONLY required if running different samples in each lane}}
{{PricingRow| | | | }}
{{PricingRow|HiSeqRapid - Additional 50 bases|full flowcell (2 lanes)|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|TruSeq ChIP DNA|Sample|$200|Optimized for low DNA input}}
{{PricingRow|TruSeq Nano gDNA|Sample|$200|Genomic DNA >100ng}}
{{PricingRow|TruSeq Stranded mRNA|Sample|$200|Basic RNA Expression Analysis}}
{{PricingRow|TruSeq Stranded RiboZero|Sample|$250|Replaces polyA selection with a ribozomal RNA depletion}}
{{PricingRow|Clontech UltraLow RNA|Sample|$400|For low input RNA down to single cell}}
{{PricingRow|Nextera genomic DNA|Sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-04-06T20:33:58Z

Volkert: /* HiSeq (Rapid) */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeqRapid - 50 bp Single Read|Run|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeqRapid - 40x40 Paired End|Run|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|Run|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeqRapid - 250x250 Paired End|Run|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeqRapid - Additional 50 bases|Run|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeqRapid - Add DUAL mode|Run|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|TruSeq ChIP DNA|Sample|$200|Optimized for low DNA input}}
{{PricingRow|TruSeq Stranded mRNA|Sample|$200|Basic RNA Expression Analysis}}
{{PricingRow|TruSeq Stranded RiboZero|Sample|$250|Replaces polyA selection with a ribozomal RNA depletion}}
{{PricingRow|Clontech UltraLow RNA|Sample|$400|For low input RNA down to single cell}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-04-06T20:31:59Z

Volkert: /* HiSeq (Rapid) */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeqRapid - 50 bp Single Read|Run (2 lanes)|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeqRapid - 40x40 Paired End|Run (2 lanes)|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeqRapid - 100x100 Paired End|Run (2 lanes)|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeqRapid - 250x250 Paired End|Run (2 lanes)|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeqRapid - Additional 50 bases|Run (2 lanes)|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeqRapid - Add DUAL mode|Run (2 lanes)|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|TruSeq ChIP DNA|Sample|$200|Optimized for low DNA input}}
{{PricingRow|TruSeq Stranded mRNA|Sample|$200|Basic RNA Expression Analysis}}
{{PricingRow|TruSeq Stranded RiboZero|Sample|$250|Replaces polyA selection with a ribozomal RNA depletion}}
{{PricingRow|Clontech UltraLow RNA|Sample|$400|For low input RNA down to single cell}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T20:50:34Z

Volkert: /* Illumina Sequencing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|TruSeq ChIP DNA|Sample|$200|Optimized for low DNA input}}
{{PricingRow|TruSeq Stranded mRNA|Sample|$200|Basic RNA Expression Analysis}}
{{PricingRow|TruSeq Stranded RiboZero|Sample|$250|Replaces polyA selection with a ribozomal RNA depletion}}
{{PricingRow|Clontech UltraLow RNA|Sample|$400|For low input RNA down to single cell}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T20:49:43Z

Volkert: /* Illumina Sequencing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the |http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services| page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|TruSeq ChIP DNA|Sample|$200|Optimized for low DNA input}}
{{PricingRow|TruSeq Stranded mRNA|Sample|$200|Basic RNA Expression Analysis}}
{{PricingRow|TruSeq Stranded RiboZero|Sample|$250|Replaces polyA selection with a ribozomal RNA depletion}}
{{PricingRow|Clontech UltraLow RNA|Sample|$400|For low input RNA down to single cell}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T20:38:38Z

Volkert: /* Library Prep */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|TruSeq ChIP DNA|Sample|$200|Optimized for low DNA input}}
{{PricingRow|TruSeq Stranded mRNA|Sample|$200|Basic RNA Expression Analysis}}
{{PricingRow|TruSeq Stranded RiboZero|Sample|$250|Replaces polyA selection with a ribozomal RNA depletion}}
{{PricingRow|Clontech UltraLow RNA|Sample|$400|For low input RNA down to single cell}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T20:31:32Z

Volkert: /* Illumina Sequencing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Sequencing

2015-01-12T20:29:52Z

Volkert: /* LIBRARY PREP SERVICE */

{{TopRightSideBar|
|title=Sequencing Service
|image=Dna_seq.jpg
|subtitle=
|highlights=

}}

= TECHNOLOGY =

The GTC offers Next-Generation sequencing on the Illumina platform. We have two HiSeq 2500 systems with Rapid mode capabilities.

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

= LIBRARY PREP SERVICE =

We offer many options for library prep for a wide range of applications and input levels down to RNA from single cells. The following chart can be used as a starting point but you should feel free to consult GTC staff about which prep is right for you.

{| class="wikitable"
|-
! scope="col"| Sample Type
! scope="col"| Application
! scope="col"| Input amount (ng)**
! scope="col"| Prep
|-
! scope="row"| Genomic DNA
| Any
| >100
| TruSeq DNA

|-
! scope="row"| ChIP
| Any
| 1-100
| TruSeq ChIP

|-
! scope="row"| RNA (total)
| Basic (PolyA) Expression
| >100
| TruSeq Stranded RNA

|-
! scope="row"| RNA (total)
| With Ribozomal Deletion
| 100-4,000
| TruSeq Stranded RNA with RiboZero

|-
! scope="row"| RNA (total)
| Ultra Low Input
| <10
| Clonetech UltraLow Input
|}

= PRICING =

For pricing information - [[Pricing| Click Here]]

{{SeqSubmissionGuide}}

= DATA =

Images acquired from the sequencer are processed through the bundled Illumina image extraction pipeline to get the sequence and quality score for each base. If requested, the reads can be aligned to a reference genome using the Illumina ELAND algorithm. An in-depth QC report is included in the package.

* QC Details - [[SequencingQC|Sequencing]]
* Formats Details - [[SequencingFormats|Sequencing]]

== TURNAROUND TIME ==

Each sequencer processes 8 lanes per run, or 7 lanes plus a control. Full flowcell submissions (7 lanes) can usually be started within one week of submission. Partial submissions of less than seven lanes are put into a project queue where they join existing samples or await others before processing. Wait times for partial submissions vary but are generally two weeks or less until the START of the run depending on the requested run configuration and demand from other users. The most common run configuration is short (40 bases) and single read. Longer read and paired-end samples may take longer to turn around as there are generally fewer similar samples in the queue to fill out runs. Once a run has started, estimate approximately 1 day per 20 bases for the run and an additional 24-48 hours for data processing. Samples requiring library prep will also take an additional one to two weeks for the prep.

= APPLICATIONS =

Next-Generation Sequencing can be used for a wide variety of applications including ChIP-Seq, RNA-Seq, smallRNA-seq, GWAS and much more. Visit Illumina's web site or contact us to discuss your project goals.

Sequencing

2015-01-12T20:29:29Z

Volkert: /* LIBRARY PREP SERVICE */

{{TopRightSideBar|
|title=Sequencing Service
|image=Dna_seq.jpg
|subtitle=
|highlights=

}}

= TECHNOLOGY =

The GTC offers Next-Generation sequencing on the Illumina platform. We have two HiSeq 2500 systems with Rapid mode capabilities.

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

= LIBRARY PREP SERVICE =

We offer many options for library prep for a wide range of applications and input levels down to RNA from single cells. The following chart can be used as a starting point but you should feel free to consult GTC staff about which prep is right for you.

{| class="wikitable"
|-
! scope="col"| Sample Type
! scope="col"| Application
! scope="col"| Input amount (ng)**
! scope="col"| Prep
|-
! scope="row"| Genomic DNA
| Any
| >100
| TruSeq DNA

|-
! scope="row"| ChIP
| Any
| 1-100
| TruSeq ChIP

|-
! scope="row"| RNA (total)
| Basic (PolyA) Expression
| >100
| TruSeq Stranded RNA

|-
! scope="row"| RNA (total)
| With Ribozomal Deletion
| 100-4,000
| TruSeq Stranded RNA with RiboZero

|-
! scope="row"| RNA (total)
| Ultra Low Input
| <10
| Clonetech UltraLow Input
|}

We now offer two options for library prep. The "Premium" service is our traditional option built on several years of optimizations we have made to the Illumina "TruSeq" library prep system. This manual protocol is optimal for low-input applications like ChIP-Seq, or RNA-Seq from small numbers of cells. Additionally we now offer a "Basic" library prep option based on the automated library prep methods of the IntegenX Apollo 324 system. This lower cost option works well for genomic DNA or more conventional RNA-Seq applications. Of note for RNA-Seq users is that the Apollo protocol is strand-specific, and offers either poly(A) mRNA isolation or ribosomal depletion to preserve non-coding RNAs. Feel free to contact us to discuss the options in more detail.

= PRICING =

For pricing information - [[Pricing| Click Here]]

{{SeqSubmissionGuide}}

= DATA =

Images acquired from the sequencer are processed through the bundled Illumina image extraction pipeline to get the sequence and quality score for each base. If requested, the reads can be aligned to a reference genome using the Illumina ELAND algorithm. An in-depth QC report is included in the package.

* QC Details - [[SequencingQC|Sequencing]]
* Formats Details - [[SequencingFormats|Sequencing]]

== TURNAROUND TIME ==

Each sequencer processes 8 lanes per run, or 7 lanes plus a control. Full flowcell submissions (7 lanes) can usually be started within one week of submission. Partial submissions of less than seven lanes are put into a project queue where they join existing samples or await others before processing. Wait times for partial submissions vary but are generally two weeks or less until the START of the run depending on the requested run configuration and demand from other users. The most common run configuration is short (40 bases) and single read. Longer read and paired-end samples may take longer to turn around as there are generally fewer similar samples in the queue to fill out runs. Once a run has started, estimate approximately 1 day per 20 bases for the run and an additional 24-48 hours for data processing. Samples requiring library prep will also take an additional one to two weeks for the prep.

= APPLICATIONS =

Next-Generation Sequencing can be used for a wide variety of applications including ChIP-Seq, RNA-Seq, smallRNA-seq, GWAS and much more. Visit Illumina's web site or contact us to discuss your project goals.

Sequencing

2015-01-12T20:27:17Z

Volkert: /* LIBRARY PREP SERVICE */

{{TopRightSideBar|
|title=Sequencing Service
|image=Dna_seq.jpg
|subtitle=
|highlights=

}}

= TECHNOLOGY =

The GTC offers Next-Generation sequencing on the Illumina platform. We have two HiSeq 2500 systems with Rapid mode capabilities.

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

= LIBRARY PREP SERVICE =

We offer many options for library prep for a wide range of applications and input levels down to RNA from single cells. The following chart can be used as a starting point but you should feel free to consult GTC staff about which prep is right for you.

{| class="wikitable"
|-
! scope="col"| Sample Type
! scope="col"| Application
! scope="col"| Input amount (ng)**
! scope="col"| Prep
|-
! scope="row"| Genomic DNA
| Any
| >100
| TruSeq DNA

|-
! scope="row"| ChIP
| Any
| 1-100
| TruSeq ChIP

|-
! scope="row"| RNA (total)
| Basic (PolyA) Expression
| >100
| TruSeq Stranded RNA

|-
! scope="row"| RNA (total)
| With Ribozomal Deletion
| 100-4,000
| TruSeq Stranded RNA with RiboZero

|-
! scope="row"| RNA (total)
| Basic (PolyA) Expression
| >100
| TruSeq Stranded RNA
|}

We now offer two options for library prep. The "Premium" service is our traditional option built on several years of optimizations we have made to the Illumina "TruSeq" library prep system. This manual protocol is optimal for low-input applications like ChIP-Seq, or RNA-Seq from small numbers of cells. Additionally we now offer a "Basic" library prep option based on the automated library prep methods of the IntegenX Apollo 324 system. This lower cost option works well for genomic DNA or more conventional RNA-Seq applications. Of note for RNA-Seq users is that the Apollo protocol is strand-specific, and offers either poly(A) mRNA isolation or ribosomal depletion to preserve non-coding RNAs. Feel free to contact us to discuss the options in more detail.

= PRICING =

For pricing information - [[Pricing| Click Here]]

{{SeqSubmissionGuide}}

= DATA =

Images acquired from the sequencer are processed through the bundled Illumina image extraction pipeline to get the sequence and quality score for each base. If requested, the reads can be aligned to a reference genome using the Illumina ELAND algorithm. An in-depth QC report is included in the package.

* QC Details - [[SequencingQC|Sequencing]]
* Formats Details - [[SequencingFormats|Sequencing]]

== TURNAROUND TIME ==

Each sequencer processes 8 lanes per run, or 7 lanes plus a control. Full flowcell submissions (7 lanes) can usually be started within one week of submission. Partial submissions of less than seven lanes are put into a project queue where they join existing samples or await others before processing. Wait times for partial submissions vary but are generally two weeks or less until the START of the run depending on the requested run configuration and demand from other users. The most common run configuration is short (40 bases) and single read. Longer read and paired-end samples may take longer to turn around as there are generally fewer similar samples in the queue to fill out runs. Once a run has started, estimate approximately 1 day per 20 bases for the run and an additional 24-48 hours for data processing. Samples requiring library prep will also take an additional one to two weeks for the prep.

= APPLICATIONS =

Next-Generation Sequencing can be used for a wide variety of applications including ChIP-Seq, RNA-Seq, smallRNA-seq, GWAS and much more. Visit Illumina's web site or contact us to discuss your project goals.

Sequencing

2015-01-12T20:20:03Z

Volkert: /* LIBRARY PREP SERVICE */

{{TopRightSideBar|
|title=Sequencing Service
|image=Dna_seq.jpg
|subtitle=
|highlights=

}}

= TECHNOLOGY =

The GTC offers Next-Generation sequencing on the Illumina platform. We have two HiSeq 2500 systems with Rapid mode capabilities.

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

= LIBRARY PREP SERVICE =

We offer many options for library prep for a wide range of applications and input levels down to RNA from single cells. The following chart can be used as a starting point but you should feel free to consult GTC staff about which prep is right for you.

We now offer two options for library prep. The "Premium" service is our traditional option built on several years of optimizations we have made to the Illumina "TruSeq" library prep system. This manual protocol is optimal for low-input applications like ChIP-Seq, or RNA-Seq from small numbers of cells. Additionally we now offer a "Basic" library prep option based on the automated library prep methods of the IntegenX Apollo 324 system. This lower cost option works well for genomic DNA or more conventional RNA-Seq applications. Of note for RNA-Seq users is that the Apollo protocol is strand-specific, and offers either poly(A) mRNA isolation or ribosomal depletion to preserve non-coding RNAs. Feel free to contact us to discuss the options in more detail.

= PRICING =

For pricing information - [[Pricing| Click Here]]

{{SeqSubmissionGuide}}

= DATA =

Images acquired from the sequencer are processed through the bundled Illumina image extraction pipeline to get the sequence and quality score for each base. If requested, the reads can be aligned to a reference genome using the Illumina ELAND algorithm. An in-depth QC report is included in the package.

* QC Details - [[SequencingQC|Sequencing]]
* Formats Details - [[SequencingFormats|Sequencing]]

== TURNAROUND TIME ==

Each sequencer processes 8 lanes per run, or 7 lanes plus a control. Full flowcell submissions (7 lanes) can usually be started within one week of submission. Partial submissions of less than seven lanes are put into a project queue where they join existing samples or await others before processing. Wait times for partial submissions vary but are generally two weeks or less until the START of the run depending on the requested run configuration and demand from other users. The most common run configuration is short (40 bases) and single read. Longer read and paired-end samples may take longer to turn around as there are generally fewer similar samples in the queue to fill out runs. Once a run has started, estimate approximately 1 day per 20 bases for the run and an additional 24-48 hours for data processing. Samples requiring library prep will also take an additional one to two weeks for the prep.

= APPLICATIONS =

Next-Generation Sequencing can be used for a wide variety of applications including ChIP-Seq, RNA-Seq, smallRNA-seq, GWAS and much more. Visit Illumina's web site or contact us to discuss your project goals.

Template:SeqSubmissionGuide

2015-01-12T20:14:03Z

Volkert: /* SUBMISSION OF SAMPLES FOR LIBRARY PREPARATION */

=SEQUENCING SUBMISSION GUIDELINES=

You can download detailed guidelines in word format from [[Media:Sample_Submission_Guide.pdf‎| here]].

==STEP 1: REGISTRATION (FOR NEW CUSTOMERS ONLY)==

* INTERNAL CUSTOMERS: Please [http://iona.wi.mit.edu/microarray/login/register.php click here] to register in our system.

* EXTERNAL CUSTOMERS: Fill out a new customer form ([http://jura.wi.mit.edu/genomecorewiki/index.php/Forms Available Here]) with billing information. Once the form is emailed to us, we will register you in our database and notify you when you can submit samples to us.
All external customers please obtain a purchase order number prior to your submission. If you need a quote, please email us at wibr-genome@wi.mit.edu or call us at 617-258-8803. Blanket purchase orders are preferred, if you plan to submit multiple projects over time.

==STEP 2: PREPARATION OF SAMPLES==

===SUBMISSION OF SAMPLES FOR LIBRARY PREPARATION===

Samples should be in a buffer that does not contain trace amounts of phenol or EDTA. We will save and return any unused input material after libraries are successfully prepared. We will archive unused library.

Below are our input requirements for Library Prep Service

{| class="wikitable"
|-
! scope="col"| Sample Type
! scope="col"| Sonication/Fragmentation*
! scope="col"| Fragment Size (bp)
! scope="col"| Input requirements (ng)**
! scope="col"| Volume (ul)
|-
! scope="row"| Genomic
| Required
| 200 to 400
| 500 to 1000
| 10-50
|-
! scope="row"| ChIP
| Required
| Variable (specify)
| 10 to 50
| 10-50
|-
! scope="row"| RNA (total)
| NA
| NA
| 100 to 1000
| 10-50
|}

<nowiki>*</nowiki>Unless special arrangements are made with us in advance, we will assume that your genomic DNA sample has been fragmented, by sonication or otherwise, prior to submission.

<nowiki>**</nowiki>We can work with less if necessary, but it is best if you elute your DNA in 50 ul or less if you believe we may need to use all of it. Please give us at least 10uL of sample to ensure we have enough for QC and prep.

===SUBMISSION OF PRE-PREPARED LIBRARIES===
We typically need 10-20 ul of each library at 5 ng/ul or greater. If your library is less concentrated than 5 ng/ul, we’ll quantify it to make sure we have enough to sequence. We will archive your leftover library.

==STEP 3: ONLINE SUBMISSION OF SEQUENCING LIBRARIES==
*Please complete the online submission form ([http://jura.wi.mit.edu/genomecorewiki/index.php/Forms Available Here]). Questions or problems related to the submission should be directed to Sumeet Gupta at sgupta@wi.mit.edu

*Every physical sample tube should have its own Genome Tech Core ID (GTC ID) number (automatically generated and emailed to you immediately after submission of the online form), even if you plan to multiplex them.

*Please write your name GTC ID numbers on the tubes. We prefer 1.5 ml tubes.

==STEP 4: DROP-OFF OR SHIPPING==
When you are ready, you may hand deliver or ship samples them on dry ice to:

Whitehead Institute Genome Technology Core 
9 Cambridge Center, Room 325 
Cambridge, MA 02142

Please do not ship without first contacting someone in the lab, to make sure that we are here to receive your package. Do not rely on voicemail. Please wait until you hear back from someone.

Sequencing

2015-01-12T20:10:32Z

Volkert: /* TECHNOLOGY */

{{TopRightSideBar|
|title=Sequencing Service
|image=Dna_seq.jpg
|subtitle=
|highlights=

}}

= TECHNOLOGY =

The GTC offers Next-Generation sequencing on the Illumina platform. We have two HiSeq 2500 systems with Rapid mode capabilities.

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

= LIBRARY PREP SERVICE =

We now offer two options for library prep. The "Premium" service is our traditional option built on several years of optimizations we have made to the Illumina "TruSeq" library prep system. This manual protocol is optimal for low-input applications like ChIP-Seq, or RNA-Seq from small numbers of cells. Additionally we now offer a "Basic" library prep option based on the automated library prep methods of the IntegenX Apollo 324 system. This lower cost option works well for genomic DNA or more conventional RNA-Seq applications. Of note for RNA-Seq users is that the Apollo protocol is strand-specific, and offers either poly(A) mRNA isolation or ribosomal depletion to preserve non-coding RNAs. Feel free to contact us to discuss the options in more detail.

= PRICING =

For pricing information - [[Pricing| Click Here]]

{{SeqSubmissionGuide}}

= DATA =

Images acquired from the sequencer are processed through the bundled Illumina image extraction pipeline to get the sequence and quality score for each base. If requested, the reads can be aligned to a reference genome using the Illumina ELAND algorithm. An in-depth QC report is included in the package.

* QC Details - [[SequencingQC|Sequencing]]
* Formats Details - [[SequencingFormats|Sequencing]]

== TURNAROUND TIME ==

Each sequencer processes 8 lanes per run, or 7 lanes plus a control. Full flowcell submissions (7 lanes) can usually be started within one week of submission. Partial submissions of less than seven lanes are put into a project queue where they join existing samples or await others before processing. Wait times for partial submissions vary but are generally two weeks or less until the START of the run depending on the requested run configuration and demand from other users. The most common run configuration is short (40 bases) and single read. Longer read and paired-end samples may take longer to turn around as there are generally fewer similar samples in the queue to fill out runs. Once a run has started, estimate approximately 1 day per 20 bases for the run and an additional 24-48 hours for data processing. Samples requiring library prep will also take an additional one to two weeks for the prep.

= APPLICATIONS =

Next-Generation Sequencing can be used for a wide variety of applications including ChIP-Seq, RNA-Seq, smallRNA-seq, GWAS and much more. Visit Illumina's web site or contact us to discuss your project goals.

Sequencing

2015-01-12T20:08:17Z

Volkert: /* TECHNOLOGY */

{{TopRightSideBar|
|title=Sequencing Service
|image=Dna_seq.jpg
|subtitle=
|highlights=

}}

= TECHNOLOGY =

The GTC offers Next-Generation sequencing on the Illumina platform. We have two HiSeq 2500 systems with Rapid mode capabilities.

All systems can be run in either Single-Read or Paired-End mode. The HiSeq standard mode typically yields 120-150 million reads per lane and requires 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

= LIBRARY PREP SERVICE =

We now offer two options for library prep. The "Premium" service is our traditional option built on several years of optimizations we have made to the Illumina "TruSeq" library prep system. This manual protocol is optimal for low-input applications like ChIP-Seq, or RNA-Seq from small numbers of cells. Additionally we now offer a "Basic" library prep option based on the automated library prep methods of the IntegenX Apollo 324 system. This lower cost option works well for genomic DNA or more conventional RNA-Seq applications. Of note for RNA-Seq users is that the Apollo protocol is strand-specific, and offers either poly(A) mRNA isolation or ribosomal depletion to preserve non-coding RNAs. Feel free to contact us to discuss the options in more detail.

= PRICING =

For pricing information - [[Pricing| Click Here]]

{{SeqSubmissionGuide}}

= DATA =

Images acquired from the sequencer are processed through the bundled Illumina image extraction pipeline to get the sequence and quality score for each base. If requested, the reads can be aligned to a reference genome using the Illumina ELAND algorithm. An in-depth QC report is included in the package.

* QC Details - [[SequencingQC|Sequencing]]
* Formats Details - [[SequencingFormats|Sequencing]]

== TURNAROUND TIME ==

Each sequencer processes 8 lanes per run, or 7 lanes plus a control. Full flowcell submissions (7 lanes) can usually be started within one week of submission. Partial submissions of less than seven lanes are put into a project queue where they join existing samples or await others before processing. Wait times for partial submissions vary but are generally two weeks or less until the START of the run depending on the requested run configuration and demand from other users. The most common run configuration is short (40 bases) and single read. Longer read and paired-end samples may take longer to turn around as there are generally fewer similar samples in the queue to fill out runs. Once a run has started, estimate approximately 1 day per 20 bases for the run and an additional 24-48 hours for data processing. Samples requiring library prep will also take an additional one to two weeks for the prep.

= APPLICATIONS =

Next-Generation Sequencing can be used for a wide variety of applications including ChIP-Seq, RNA-Seq, smallRNA-seq, GWAS and much more. Visit Illumina's web site or contact us to discuss your project goals.

Tom Volkert

2015-01-12T20:04:37Z

Volkert:

{| style="text-align: left; width:90%"
|
[[image:Tom.jpg|right]]
[[Tom_Volkert| Tom Volkert]] 
Director, 
Phone: 617-258-5094 
Email: volkert at wi dot mit dot edu
|}

Personnel

2015-01-12T20:04:07Z

Volkert:

{| style="background:white; color:black; width:800px" align="center"
|
{| style="border:1px solid purple; width:400px; height:150px; text-align: center"
|
{| style="text-align: left; width:90%"
|
[[image:Tom.jpg|right]]
[[Tom_Volkert| Tom Volkert]] 
Director, 
Phone: 617-258-5094 
Email: volkert at wi dot mit dot edu
|}
|}
||
{| style="border:1px solid purple; width:400px; height:150px; text-align: center"
|
{| style="text-align: left; width:90%"
|
[[image:Jen.jpg|right]]
[[Jennifer_Love| Jennifer Love]] 
Technical Associate II, 
Phone: 617-258-8803 
Email: jlove at wi dot mit dot edu 
|}
|}
|
|}
{| style="background:white; color:black; width:800px" align="center"
|
{| style="border:1px solid purple; width:400px; height:150px; text-align: center"
|
{| style="text-align: left; width:90%"
|
[[image:Sumeet.jpg|right]]
[[Sumeet_Gupta| Sumeet Gupta]] 
Bioinformatics Analyst, 
Phone: 617-324-0339 
Email: sgupta at wi dot mit dot edu 
|}
|}
||
{| style="border:1px solid purple; width:400px; height:150px; text-align: center"
|
{| style="text-align: left; width:90%"
|
[[image:lakesha.jpg|right]]
[[La'kesha Francis| La'kesha Francis]] 
Technical Assistant I, 
Phone: 617-258-8803 
Email: lfrancis at wi dot mit dot edu 
|}
|}
|
|}
{| style="border:1px solid purple; width:400px; height:150px; text-align: center" align="center"
|
{| style="text-align: left; width:90%"
|
[[image:stephen.jpg|right]]
[[Stephen Mraz III| Stephen Mraz III]] 
Technical Assistant I, 
Phone: 617-258-8803 
Email: sjmraz3 at wi dot mit dot edu 
|}
|}

Pricing

2015-01-12T19:59:43Z

Volkert: /* Illumina Sequencing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T19:52:58Z

Volkert: /* GAII */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T19:51:26Z

Volkert: /* Agilent Array Processing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|Array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T19:50:58Z

Volkert: /* Agilent Array Processing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T19:49:59Z

Volkert: /* Agilent Array Processing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|$525|Not including cost of array}}
{{PricingLastRow|Contact GTC or Agilent for Array pricing|array|$$|}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T19:46:36Z

Volkert: /* Affymetrix Array Processing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T19:45:43Z

Volkert: /* Affymetrix Array Processing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
{{PricingRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}
{{PricingLastRow|}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T19:40:01Z

Volkert: /* HiSeq (Rapid) */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Affymetrix Gene ST or PrimeView arrays |Array|~$175|Array only}}
{{PricingRow|Affymetrix Genome (U133 or 430) 2.0 |Array|~$400|Array only}}
{{PricingRow|Standard Expression Processing|Sample|~$500|all Affymetrix certified reagents}}
{{PricingLastRow|Prokaryotic Protocol|Sample|~$450|-}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T19:39:03Z

Volkert: /* HiSeq (Rapid) */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 150x150}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 

=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Affymetrix Gene ST or PrimeView arrays |Array|~$175|Array only}}
{{PricingRow|Affymetrix Genome (U133 or 430) 2.0 |Array|~$400|Array only}}
{{PricingRow|Standard Expression Processing|Sample|~$500|all Affymetrix certified reagents}}
{{PricingLastRow|Prokaryotic Protocol|Sample|~$450|-}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2015-01-12T19:33:20Z

Volkert: /* HiSeq (Std) */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 

=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,700|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 50x50 Paired End|Sample|$2,500|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,240|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 150x150}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 
=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Affymetrix Gene ST or PrimeView arrays |Array|~$175|Array only}}
{{PricingRow|Affymetrix Genome (U133 or 430) 2.0 |Array|~$400|Array only}}
{{PricingRow|Standard Expression Processing|Sample|~$500|all Affymetrix certified reagents}}
{{PricingLastRow|Prokaryotic Protocol|Sample|~$450|-}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2013-08-13T14:09:12Z

Volkert: /* Library Prep */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,060|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,575|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,100|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,475|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 
=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,700|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,240|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 150x150}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 
=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Affymetrix Gene ST or PrimeView arrays |Array|~$175|Array only}}
{{PricingRow|Affymetrix Genome (U133 or 430) 2.0 |Array|~$400|Array only}}
{{PricingRow|Standard Expression Processing|Sample|~$500|all Affymetrix certified reagents}}
{{PricingLastRow|Prokaryotic Protocol|Sample|~$450|-}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2013-08-13T14:08:35Z

Volkert: /* Library Prep */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq (Std) ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,060|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,575|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,100|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,475|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 
=== HiSeq (Rapid) ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,700|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,240|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 150x150}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 
=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly A mRNA isolation (add to library prep cost)}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 

=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Affymetrix Gene ST or PrimeView arrays |Array|~$175|Array only}}
{{PricingRow|Affymetrix Genome (U133 or 430) 2.0 |Array|~$400|Array only}}
{{PricingRow|Standard Expression Processing|Sample|~$500|all Affymetrix certified reagents}}
{{PricingLastRow|Prokaryotic Protocol|Sample|~$450|-}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}

Pricing

2013-08-09T17:52:22Z

Volkert: /* Illumina Sequencing */

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

= Illumina Sequencing =

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

=== GAII ===
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
 
=== HiSeq ===
{{PricingHeader}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,060|avg 120-130 million reads}}
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,575|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,100|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,475|avg 120-130 million paired end reads}}
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}

{{PricingLastRow||||}}
 
=== HiSeq 2500 ===
{{PricingHeader}}
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,700|avg 100-120 million reads}}
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,240|avg 100-120 million paired end reads}}
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 150x150}}
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
{{PricingLastRow||||}}

 
=== Library Prep ===
{{PricingHeader}}
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly A mRNA isolation}}
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 
=== Quality Control ===
{{PricingHeader}}
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
{{PricingRow|qPCR|Sample|$25|Quantification}}
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 

= Microarray Services =

Prices for sample processing do not include cost of arrays

== Affymetrix Array Processing==
{{PricingHeader}}
{{PricingRow|Affymetrix Gene ST or PrimeView arrays |Array|~$175|Array only}}
{{PricingRow|Affymetrix Genome (U133 or 430) 2.0 |Array|~$400|Array only}}
{{PricingRow|Standard Expression Processing|Sample|~$500|all Affymetrix certified reagents}}
{{PricingLastRow|Prokaryotic Protocol|Sample|~$450|-}}

 

== Agilent Array Processing==
{{PricingHeader}}
{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}

 

= nCounter Analysis System (NanoString) =
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}

 
= Bioanalyzer - RNA, DNA and Protein analysis =
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}

 

= Quantitative PCR - ABI 7900 =
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}